A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments

Handle URI:
http://hdl.handle.net/10754/325447
Title:
A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments
Authors:
Rodrigo, María J.; Alquézar, Berta; Alós, Enriqueta; Medina, Víctor; Carmona, Lourdes; Bruno, Mark; Al-Babili, Salim ( 0000-0003-4823-2882 ) ; Zacarías, Lorenzo
Abstract:
Citrus is the first tree crop in terms of fruit production. The colour of Citrus fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C30 apocarotenoids, mainly ?-citraurin (3-hydroxy-?-apo-8?-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and mandarins. Though carotenoid biosynthesis and its regulation have been extensively studied in Citrus fruits, little is known about the formation of C30 apocarotenoids. The aim of this study was to the identify carotenoid cleavage enzyme(s) [CCD(s)] involved in the peel-specific C30 apocarotenoids. In silico data mining revealed a new family of five CCD4-type genes in Citrus. One gene of this family, CCD4b1, was expressed in reproductive and vegetative tissues of different Citrus species in a pattern correlating with the accumulation of C30 apocarotenoids. Moreover, developmental processes and treatments which alter Citrus fruit peel pigmentation led to changes of ?-citraurin content and CCD4b1 transcript levels. These results point to the involvement of CCD4b1 in ?-citraurin formation and indicate that the accumulation of this compound is determined by the availability of the presumed precursors zeaxanthin and ?-cryptoxanthin. Functional analysis of CCD4b1 by in vitro assays unequivocally demonstrated the asymmetric cleavage activity at the 7?,8? double bond in zeaxanthin and ?-cryptoxanthin, confrming its role in C30 apocarotenoid biosynthesis. Thus, a novel plant carotenoid cleavage activity targeting the 7?,8? double bond of cyclic C40 carotenoids has been identified. These results suggest that the presented enzyme is responsible for the biosynthesis of C30 apocarotenoids in Citrus which are key pigments in fruit coloration. The Author 2013.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Rodrigo MJ, Alquezar B, Alos E, Medina V, Carmona L, et al. (2013) A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments. Journal of Experimental Botany 64: 4461-4478. doi:10.1093/jxb/ert260.
Publisher:
Oxford University Press (OUP)
Journal:
Journal of Experimental Botany
Issue Date:
4-Sep-2013
DOI:
10.1093/jxb/ert260
PubMed ID:
24006419
PubMed Central ID:
PMC3808326
Type:
Article
ISSN:
00220957
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorRodrigo, María J.en
dc.contributor.authorAlquézar, Bertaen
dc.contributor.authorAlós, Enriquetaen
dc.contributor.authorMedina, Víctoren
dc.contributor.authorCarmona, Lourdesen
dc.contributor.authorBruno, Marken
dc.contributor.authorAl-Babili, Salimen
dc.contributor.authorZacarías, Lorenzoen
dc.date.accessioned2014-08-27T09:51:44Z-
dc.date.available2014-08-27T09:51:44Z-
dc.date.issued2013-09-04en
dc.identifier.citationRodrigo MJ, Alquezar B, Alos E, Medina V, Carmona L, et al. (2013) A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments. Journal of Experimental Botany 64: 4461-4478. doi:10.1093/jxb/ert260.en
dc.identifier.issn00220957en
dc.identifier.pmid24006419en
dc.identifier.doi10.1093/jxb/ert260en
dc.identifier.urihttp://hdl.handle.net/10754/325447en
dc.description.abstractCitrus is the first tree crop in terms of fruit production. The colour of Citrus fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C30 apocarotenoids, mainly ?-citraurin (3-hydroxy-?-apo-8?-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and mandarins. Though carotenoid biosynthesis and its regulation have been extensively studied in Citrus fruits, little is known about the formation of C30 apocarotenoids. The aim of this study was to the identify carotenoid cleavage enzyme(s) [CCD(s)] involved in the peel-specific C30 apocarotenoids. In silico data mining revealed a new family of five CCD4-type genes in Citrus. One gene of this family, CCD4b1, was expressed in reproductive and vegetative tissues of different Citrus species in a pattern correlating with the accumulation of C30 apocarotenoids. Moreover, developmental processes and treatments which alter Citrus fruit peel pigmentation led to changes of ?-citraurin content and CCD4b1 transcript levels. These results point to the involvement of CCD4b1 in ?-citraurin formation and indicate that the accumulation of this compound is determined by the availability of the presumed precursors zeaxanthin and ?-cryptoxanthin. Functional analysis of CCD4b1 by in vitro assays unequivocally demonstrated the asymmetric cleavage activity at the 7?,8? double bond in zeaxanthin and ?-cryptoxanthin, confrming its role in C30 apocarotenoid biosynthesis. Thus, a novel plant carotenoid cleavage activity targeting the 7?,8? double bond of cyclic C40 carotenoids has been identified. These results suggest that the presented enzyme is responsible for the biosynthesis of C30 apocarotenoids in Citrus which are key pigments in fruit coloration. The Author 2013.en
dc.language.isoenen
dc.publisherOxford University Press (OUP)en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0en
dc.subject?-citraurinen
dc.subjectApocarotenoiden
dc.subjectCarotenoiden
dc.subjectCarotenoid cleavage dioxygenaseen
dc.subjectCitrusen
dc.subjectFruit colorationen
dc.subjectcarotenoiden
dc.subjectethyleneen
dc.subjectethylene derivativeen
dc.subjectmessenger RNAen
dc.subjectvegetable proteinen
dc.subjectamino acid sequenceen
dc.subjectantibody specificityen
dc.subjectbiosynthesisen
dc.subjectcarotenoid cleavage dioxygenaseen
dc.subjectchemical structureen
dc.subjectchemistryen
dc.subjectdrug effecten
dc.subjectfruit coloration.en
dc.subjectgene expression regulationen
dc.subjectgeneticsen
dc.subjectheaten
dc.subjecthigh performance liquid chromatographyen
dc.subjectmetabolismen
dc.subjectmolecular geneticsen
dc.subjectmultigene familyen
dc.subjectplant geneen
dc.subjectreproductionen
dc.subjectsequence alignmenten
dc.subjectcarotenoiden
dc.subjectcarotenoid cleavage dioxygenaseen
dc.subjectfruit coloration.en
dc.subjectAmino Acid Sequenceen
dc.subjectCarotenoidsen
dc.subjectChromatography, High Pressure Liquiden
dc.subjectEthylenesen
dc.subjectGene Expression Regulation, Planten
dc.subjectGenes, Planten
dc.subjectHot Temperatureen
dc.subjectModels, Molecularen
dc.subjectMolecular Sequence Dataen
dc.subjectMultigene Familyen
dc.subjectOrgan Specificityen
dc.subjectPlant Proteinsen
dc.subjectReproductionen
dc.subjectRNA, Messengeren
dc.subjectSequence Alignmenten
dc.titleA novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigmentsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalJournal of Experimental Botanyen
dc.identifier.pmcidPMC3808326en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionInstituto de Agroqumica y Tecnologa de Alimentos, Consejo Superior de Investigaciones Cientficas (IATA-CSIC), Av. Agustn Escardino 7, 46980 Paterna, Valencia, Spainen
dc.contributor.institutionFaculty of Biology, University of Freiburg, D-79104 Freiburg, Germanyen
dc.contributor.institutionCentro de Proteccin Vegetal y Biotecnologa, Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Nquera, 46113, Moncada, Valencia, Spainen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAl-Babili, Salimen

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