Identification of distinct quantitative trait loci associated with defence against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula

Handle URI:
http://hdl.handle.net/10754/325446
Title:
Identification of distinct quantitative trait loci associated with defence against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula
Authors:
Guo, Su-Min; Kamphuis, Lars G.; Gao, Ling-Ling; Klingler, John P.; Lichtenzveig, Judith; Edwards, Owain; Singh, Karam B.
Abstract:
Aphids are a major family of plant insect pests. Medicago truncatula and Acyrthosiphon pisum (pea aphid, PA) are model species with a suite of resources available to help dissect the mechanism underlying plant-aphid interactions. A previous study focused on monogenic and relatively strong resistance in M. truncatula to PA and other aphid species. In this study a moderate resistance to PA was characterized in detail in the M. truncatula line A17 and compared with the highly susceptible line A20 and the more resistant line Jester. The results show that PA resistance in A17 involves both antibiosis and tolerance, and that resistance is phloem based. Quantitative trait locus (QTL) analysis using a recombinant inbred line (RIL) population (n=114) from a cross between A17 and A20 revealed that one locus, which co-segregated with AIN (Acyrthosiphon-induced necrosis) on chromosome 3, is responsible for the reduction of aphid biomass (indicator of antibiosis) for both PA and bluegreen aphid (BGA, A. kondoi), albeit to a lesser degree for PA than BGA. Interestingly, two independent loci on chromosomes 5 and 3 were identified for the plant biomass reduction (indicator of plant tolerance) by PA and BGA, respectively, demonstrating that the plant's tolerance response to these two closely related aphid species is distinct. Together with previously identified major resistant (R) genes, the QTLs identified in this study are powerful tools to understand fully the spectrum of plant defence against sap-sucking insects and provide opportunities for breeders to generate effective and sustainable strategies for aphid control. 2012 The Author.
KAUST Department:
Plant Stress Genomics Research Lab
Citation:
Guo S-M, Kamphuis LG, Gao L-L, Klingler JP, Lichtenzveig J, et al. (2012) Identification of distinct quantitative trait loci associated with defence against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula. Journal of Experimental Botany 63: 3913-3922. doi:10.1093/jxb/ers084.
Publisher:
Oxford University Press
Journal:
Journal of Experimental Botany
Issue Date:
21-Mar-2012
DOI:
10.1093/jxb/ers084
PubMed ID:
22442407
PubMed Central ID:
PMC3388833
Type:
Article
ISSN:
00220957
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGuo, Su-Minen
dc.contributor.authorKamphuis, Lars G.en
dc.contributor.authorGao, Ling-Lingen
dc.contributor.authorKlingler, John P.en
dc.contributor.authorLichtenzveig, Judithen
dc.contributor.authorEdwards, Owainen
dc.contributor.authorSingh, Karam B.en
dc.date.accessioned2014-08-27T09:51:41Z-
dc.date.available2014-08-27T09:51:41Z-
dc.date.issued2012-03-21en
dc.identifier.citationGuo S-M, Kamphuis LG, Gao L-L, Klingler JP, Lichtenzveig J, et al. (2012) Identification of distinct quantitative trait loci associated with defence against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula. Journal of Experimental Botany 63: 3913-3922. doi:10.1093/jxb/ers084.en
dc.identifier.issn00220957en
dc.identifier.pmid22442407en
dc.identifier.doi10.1093/jxb/ers084en
dc.identifier.urihttp://hdl.handle.net/10754/325446en
dc.description.abstractAphids are a major family of plant insect pests. Medicago truncatula and Acyrthosiphon pisum (pea aphid, PA) are model species with a suite of resources available to help dissect the mechanism underlying plant-aphid interactions. A previous study focused on monogenic and relatively strong resistance in M. truncatula to PA and other aphid species. In this study a moderate resistance to PA was characterized in detail in the M. truncatula line A17 and compared with the highly susceptible line A20 and the more resistant line Jester. The results show that PA resistance in A17 involves both antibiosis and tolerance, and that resistance is phloem based. Quantitative trait locus (QTL) analysis using a recombinant inbred line (RIL) population (n=114) from a cross between A17 and A20 revealed that one locus, which co-segregated with AIN (Acyrthosiphon-induced necrosis) on chromosome 3, is responsible for the reduction of aphid biomass (indicator of antibiosis) for both PA and bluegreen aphid (BGA, A. kondoi), albeit to a lesser degree for PA than BGA. Interestingly, two independent loci on chromosomes 5 and 3 were identified for the plant biomass reduction (indicator of plant tolerance) by PA and BGA, respectively, demonstrating that the plant's tolerance response to these two closely related aphid species is distinct. Together with previously identified major resistant (R) genes, the QTLs identified in this study are powerful tools to understand fully the spectrum of plant defence against sap-sucking insects and provide opportunities for breeders to generate effective and sustainable strategies for aphid control. 2012 The Author.en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0en
dc.subjectAntibiosisen
dc.subjectantixenosisen
dc.subjectEPGen
dc.subjectherbivoryen
dc.subjecthypersensitive responseen
dc.subjectnecrosisen
dc.subjectphloemen
dc.subjectsap-sucking insecten
dc.subjectvegetable proteinen
dc.subjectaphiden
dc.subjectbarrel medicen
dc.subjectchromosome mapen
dc.subjectgeneticsen
dc.subjectimmunologyen
dc.subjectparasitologyen
dc.subjectphysiologyen
dc.subjectplant diseaseen
dc.subjectquantitative trait locusen
dc.subjectAphidsen
dc.subjectChromosome Mappingen
dc.subjectMedicago truncatulaen
dc.subjectPlant Diseasesen
dc.subjectPlant Proteinsen
dc.subjectQuantitative Trait Locien
dc.subjectAcyrthosiphonen
dc.subjectAcyrthosiphon kondoien
dc.subjectAcyrthosiphon pisumen
dc.subjectAphididaeen
dc.subjectHexapodaen
dc.subjectMedicago truncatulaen
dc.titleIdentification of distinct quantitative trait loci associated with defence against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatulaen
dc.typeArticleen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalJournal of Experimental Botanyen
dc.identifier.pmcidPMC3388833en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionKey Laboratory of Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, Chinaen
dc.contributor.institutionCSIRO Plant Industry, Private Bag 5, Wembley, WA 6913, Australiaen
dc.contributor.institutionUWA Institute of Agriculture, University of Western Australia, Crawley, WA 6009, Australiaen
dc.contributor.institutionCSIRO Entomology, Private Bag 5, Wembley, WA 6913, Australiaen
dc.contributor.institutionAustralian Centre for Necrotrophic Fungal Pathogens, Department of Environment and Agriculture, Curtin University, Bentley, WA 6102, Australiaen
dc.contributor.institutionCSIRO Ecosystem Sciences, Private Bag 5, Wembley, WA 6913, Australiaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKlingler, Johnen

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