YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana

Handle URI:
http://hdl.handle.net/10754/325445
Title:
YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana
Authors:
Kim, Jeong Im; Murphy, Angus S.; Baek, Dongwon; Lee, Shin-Woo; Yun, Dae-Jin; Bressan, Ray A.; Narasimhan, Meena L.
Abstract:
The Arabidopsis thaliana YUCCA family of flavin monooxygenase proteins catalyses a rate-limiting step in de novo auxin biosynthesis. A YUCCA6 activation mutant, yuc6-1D, has been shown to contain an elevated free IAA level and to display typical high-auxin phenotypes. It is reported here that Arabidopsis plants over-expressing YUCCA6, such as the yuc6-1D activation mutant and 35S:YUC6 transgenic plants, displayed dramatic longevity. In addition, plants over-expressing YUCCA6 exhibited classical, delayed dark-induced and hormone-induced senescence in assays using detached rosette leaves. However, plants over-expressing an allele of YUCCA6, that carries mutations in the NADPH cofactor binding site, exhibited neither delayed leaf senescence phenotypes nor phenotypes typical of auxin overproduction. When the level of free IAA was reduced in yuc6-1D by conjugation to lysine, yuc6-1D leaves senesced at a rate similar to the wild-type leaves. Dark-induced senescence in detached leaves was accompanied by a decrease in their free IAA content, by the reduced expression of auxin biosynthesis enzymes such as YUCCA1 and YUCCA6 that increase cellular free IAA levels, and by the increased expression of auxin-conjugating enzymes encoded by the GH3 genes that reduce the cellular free auxin levels. Reduced transcript abundances of SAG12, NAC1, and NAC6 during senescence in yuc6-1D compared with the wild type suggested that auxin delays senescence by directly or indirectly regulating the expression of senescence-associated genes. 2011 The Author(s).
KAUST Department:
Plant Stress Genomics Research Lab
Citation:
Kim JI, Murphy AS, Baek D, Lee S-W, Yun D-J, et al. (2011) YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana. Journal of Experimental Botany 62: 3981-3992. doi:10.1093/jxb/err094.
Publisher:
Oxford University Press (OUP)
Journal:
Journal of Experimental Botany
Issue Date:
21-Apr-2011
DOI:
10.1093/jxb/err094
PubMed ID:
21511905
PubMed Central ID:
PMC3134353
Type:
Article
ISSN:
00220957
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorKim, Jeong Imen
dc.contributor.authorMurphy, Angus S.en
dc.contributor.authorBaek, Dongwonen
dc.contributor.authorLee, Shin-Wooen
dc.contributor.authorYun, Dae-Jinen
dc.contributor.authorBressan, Ray A.en
dc.contributor.authorNarasimhan, Meena L.en
dc.date.accessioned2014-08-27T09:51:38Z-
dc.date.available2014-08-27T09:51:38Z-
dc.date.issued2011-04-21en
dc.identifier.citationKim JI, Murphy AS, Baek D, Lee S-W, Yun D-J, et al. (2011) YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana. Journal of Experimental Botany 62: 3981-3992. doi:10.1093/jxb/err094.en
dc.identifier.issn00220957en
dc.identifier.pmid21511905en
dc.identifier.doi10.1093/jxb/err094en
dc.identifier.urihttp://hdl.handle.net/10754/325445en
dc.description.abstractThe Arabidopsis thaliana YUCCA family of flavin monooxygenase proteins catalyses a rate-limiting step in de novo auxin biosynthesis. A YUCCA6 activation mutant, yuc6-1D, has been shown to contain an elevated free IAA level and to display typical high-auxin phenotypes. It is reported here that Arabidopsis plants over-expressing YUCCA6, such as the yuc6-1D activation mutant and 35S:YUC6 transgenic plants, displayed dramatic longevity. In addition, plants over-expressing YUCCA6 exhibited classical, delayed dark-induced and hormone-induced senescence in assays using detached rosette leaves. However, plants over-expressing an allele of YUCCA6, that carries mutations in the NADPH cofactor binding site, exhibited neither delayed leaf senescence phenotypes nor phenotypes typical of auxin overproduction. When the level of free IAA was reduced in yuc6-1D by conjugation to lysine, yuc6-1D leaves senesced at a rate similar to the wild-type leaves. Dark-induced senescence in detached leaves was accompanied by a decrease in their free IAA content, by the reduced expression of auxin biosynthesis enzymes such as YUCCA1 and YUCCA6 that increase cellular free IAA levels, and by the increased expression of auxin-conjugating enzymes encoded by the GH3 genes that reduce the cellular free auxin levels. Reduced transcript abundances of SAG12, NAC1, and NAC6 during senescence in yuc6-1D compared with the wild type suggested that auxin delays senescence by directly or indirectly regulating the expression of senescence-associated genes. 2011 The Author(s).en
dc.language.isoenen
dc.publisherOxford University Press (OUP)en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)en
dc.subjectArabidopsis thalianaen
dc.subjectauxinen
dc.subjectleaf senescenceen
dc.subjectlongevityen
dc.subjectYUCCA6en
dc.subjectArabidopsis proteinen
dc.subjectindoleacetic aciden
dc.subjectindoleacetic acid derivativeen
dc.subjectmixed function oxidaseen
dc.subjectphytohormoneen
dc.subjectYUCCA6 protein, Arabidopsisen
dc.subjectArabidopsisen
dc.subjectdominant geneen
dc.subjectgene expression regulationen
dc.subjectgeneticsen
dc.subjectgrowth, development and agingen
dc.subjectmetabolismen
dc.subjectmutationen
dc.subjectplant leafen
dc.subjecttransgenic planten
dc.subjectArabidopsisen
dc.subjectArabidopsis Proteinsen
dc.subjectGene Expression Regulation, Planten
dc.subjectGenes, Dominanten
dc.subjectIndoleacetic Acidsen
dc.subjectMixed Function Oxygenasesen
dc.subjectMutagenesis, Insertionalen
dc.subjectMutationen
dc.subjectPlant Growth Regulatorsen
dc.subjectPlant Leavesen
dc.subjectPlants, Genetically Modifieden
dc.subjectArabidopsisen
dc.titleYUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thalianaen
dc.typeArticleen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalJournal of Experimental Botanyen
dc.identifier.pmcidPMC3134353en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907-2010, United Statesen
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-2010, United Statesen
dc.contributor.institutionDivision of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, South Koreaen
dc.contributor.institutionDepartment of Agronomy and Medicinal Plant Resources, Gyeongnam National University of Science and Technology, Jinju 660-758, South Koreaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorBressan, Ray Anthonyen
kaust.authorNarasimhan, Meena L.en

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