Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor

Handle URI:
http://hdl.handle.net/10754/325370
Title:
Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor
Authors:
Nagashima, Yukihiro; Mishiba, Kei-ichiro; Suzuki, Eiji; Shimada, Yukihisa; Iwata, Yuji; Koizumi, Nozomu
Abstract:
IRE1 plays an essential role in the endoplasmic reticulum (ER) stress response in yeast and mammals. We found that a double mutant of Arabidopsis IRE1A and IRE1B (ire1a/ire1b) is more sensitive to the ER stress inducer tunicamycin than the wild-type. Transcriptome analysis revealed that genes whose induction was reduced in ire1a/ire1b largely overlapped those in the bzip60 mutant. We observed that the active form of bZIP60 protein detected in the wild-type was missing in ire1a/ire1b. We further demonstrated that bZIP60 mRNA is spliced by ER stress, removing 23 ribonucleotides and therefore causing a frameshift that replaces the C-terminal region of bZIP60 including the transmembrane domain (TMD) with a shorter region without a TMD. This splicing was detected in ire1a and ire1b single mutants, but not in the ire1a/ire1b double mutant. We conclude that IRE1A and IRE1B catalyse unconventional splicing of bZIP60 mRNA to produce the active transcription factor.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Nagashima Y, Mishiba K, Suzuki E, Shimada Y, Iwata Y, et al. (2011) Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor. Sci Rep 1. doi:10.1038/srep00029.
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
1-Jul-2011
DOI:
10.1038/srep00029
PubMed ID:
22355548
PubMed Central ID:
PMC3216516
Type:
Article
ISSN:
20452322
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorNagashima, Yukihiroen
dc.contributor.authorMishiba, Kei-ichiroen
dc.contributor.authorSuzuki, Eijien
dc.contributor.authorShimada, Yukihisaen
dc.contributor.authorIwata, Yujien
dc.contributor.authorKoizumi, Nozomuen
dc.date.accessioned2014-08-27T09:49:32Z-
dc.date.available2014-08-27T09:49:32Z-
dc.date.issued2011-07-01en
dc.identifier.citationNagashima Y, Mishiba K, Suzuki E, Shimada Y, Iwata Y, et al. (2011) Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor. Sci Rep 1. doi:10.1038/srep00029.en
dc.identifier.issn20452322en
dc.identifier.pmid22355548en
dc.identifier.doi10.1038/srep00029en
dc.identifier.urihttp://hdl.handle.net/10754/325370en
dc.description.abstractIRE1 plays an essential role in the endoplasmic reticulum (ER) stress response in yeast and mammals. We found that a double mutant of Arabidopsis IRE1A and IRE1B (ire1a/ire1b) is more sensitive to the ER stress inducer tunicamycin than the wild-type. Transcriptome analysis revealed that genes whose induction was reduced in ire1a/ire1b largely overlapped those in the bzip60 mutant. We observed that the active form of bZIP60 protein detected in the wild-type was missing in ire1a/ire1b. We further demonstrated that bZIP60 mRNA is spliced by ER stress, removing 23 ribonucleotides and therefore causing a frameshift that replaces the C-terminal region of bZIP60 including the transmembrane domain (TMD) with a shorter region without a TMD. This splicing was detected in ire1a and ire1b single mutants, but not in the ire1a/ire1b double mutant. We conclude that IRE1A and IRE1B catalyse unconventional splicing of bZIP60 mRNA to produce the active transcription factor.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en
dc.subjectArabidopsis proteinen
dc.subjectbasic leucine zipper transcription factoren
dc.subjectbZIP60 protein, Arabidopsisen
dc.subjectIre1 2 protein, Arabidopsisen
dc.subjectIre1-2 protein, Arabidopsisen
dc.subjectmessenger RNAen
dc.subjectprotein kinaseen
dc.subjecttranscription factoren
dc.subjecttranscriptomeen
dc.subjecttunicamycinen
dc.subjectArabidopsisen
dc.subjectbiocatalysisen
dc.subjectgeneticsen
dc.subjectmetabolismen
dc.subjectpolymerase chain reactionen
dc.subjectRNA splicingen
dc.subjectArabidopsisen
dc.subjectArabidopsis Proteinsen
dc.subjectBasic-Leucine Zipper Transcription Factorsen
dc.subjectBiocatalysisen
dc.subjectPolymerase Chain Reactionen
dc.subjectProtein Kinasesen
dc.subjectRNA Splicingen
dc.subjectRNA, Messengeren
dc.subjectTranscription Factorsen
dc.subjectTranscriptomeen
dc.subjectTunicamycinen
dc.titleArabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factoren
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.identifier.pmcidPMC3216516en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionGraduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Nakaku, Sakai, Osaka 599-8531, Japanen
dc.contributor.institutionKihara Institute for Biological Research, Yokohama City University, Maiokacho 641-12, Totsuka, Yokohama Kanagawa 244-0813, Japanen
dc.contributor.institutionBiology Department, Huck Institutes of the Life Sciences, Pennsylvania State University, 211 Wartik, University Park, PA 16802, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorIwata, Yujien

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