Actinomycetes from red sea sponges: Sources for chemical and phylogenetic diversity

Handle URI:
http://hdl.handle.net/10754/325360
Title:
Actinomycetes from red sea sponges: Sources for chemical and phylogenetic diversity
Authors:
Abdelmohsen, Usama Ramadan; Yang, Chen; Horn, Hannes; Hajjar, Dina ( 0000-0003-3901-1172 ) ; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Hentschel, Ute
Abstract:
The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery. 2014 by the authors; licensee MDPI.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Citation:
Abdelmohsen U, Yang C, Horn H, Hajjar D, Ravasi T, et al. (2014) Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity. Marine Drugs 12: 2771-2789. doi:10.3390/md12052771.
Publisher:
MDPI AG
Journal:
Marine Drugs
Issue Date:
12-May-2014
DOI:
10.3390/md12052771
PubMed ID:
24824024
PubMed Central ID:
PMC4052315
Type:
Article
ISSN:
16603397
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAbdelmohsen, Usama Ramadanen
dc.contributor.authorYang, Chenen
dc.contributor.authorHorn, Hannesen
dc.contributor.authorHajjar, Dinaen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorHentschel, Uteen
dc.date.accessioned2014-08-27T09:49:05Z-
dc.date.available2014-08-27T09:49:05Z-
dc.date.issued2014-05-12en
dc.identifier.citationAbdelmohsen U, Yang C, Horn H, Hajjar D, Ravasi T, et al. (2014) Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity. Marine Drugs 12: 2771-2789. doi:10.3390/md12052771.en
dc.identifier.issn16603397en
dc.identifier.pmid24824024en
dc.identifier.doi10.3390/md12052771en
dc.identifier.urihttp://hdl.handle.net/10754/325360en
dc.description.abstractThe diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery. 2014 by the authors; licensee MDPI.en
dc.language.isoenen
dc.publisherMDPI AGen
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).en
dc.subjectActinomycetesen
dc.subjectBioactivityen
dc.subjectNRPSen
dc.subjectPKS Ien
dc.subjectPKS IIen
dc.subjectRed seaen
dc.subjectSpongesen
dc.subjectnatural producten
dc.subjectnonribosomal peptide synthetaseen
dc.subjectpolyketide synthaseen
dc.subjectpolyketide synthase 1en
dc.subjectpolyketide synthase 2en
dc.subjectRNA 16Sen
dc.subjectunclassified drugen
dc.subject16S rRNA geneen
dc.subjectActinobacteriaen
dc.subjectantibacterial activityen
dc.subjectantifungal activityen
dc.subjectantileishmanial activityen
dc.subjectantimicrobial activityen
dc.subjectantitrypanosomal activityen
dc.subjectBacillusen
dc.subjectbacterium identificationen
dc.subjectbacterium isolateen
dc.subjectbacterium isolationen
dc.subjectcontrolled studyen
dc.subjectdrug screeningen
dc.subjectenzymatic assayen
dc.subjectEscherichia coli K 12en
dc.subjectFusariumen
dc.subjectgene sequenceen
dc.subjectgenetic variabilityen
dc.subjectGram negative bacteriumen
dc.subjectGram positive bacteriumen
dc.subjectKocuriaen
dc.subjectLeishmania majoren
dc.subjectMycobacteriumen
dc.subjectNocardiaen
dc.subjectnucleotide sequenceen
dc.subjectphylogenyen
dc.subjectpolymerase chain reactionen
dc.subjectRed sea spongeen
dc.subjectRhodococcusen
dc.subjectSaudi Arabiaen
dc.subjectsequence homologyen
dc.subjectspecies cultivationen
dc.subjectsponge (Porifera)en
dc.subjectTrypanosoma bruceien
dc.subjectWest Nile flavivirusen
dc.titleActinomycetes from red sea sponges: Sources for chemical and phylogenetic diversityen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalMarine Drugsen
dc.identifier.pmcidPMC4052315en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Wrzburg, Julius-von-Sachs-Platz 3, Wrzburg D-97082, Germanyen
dc.contributor.institutionDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypten
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorYang, Chenen
kaust.authorRavasi, Timothyen
kaust.authorHajjar, Dinaen

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