Role of RNA interference (RNAi) in the moss Physcomitrella patens

Handle URI:
http://hdl.handle.net/10754/325355
Title:
Role of RNA interference (RNAi) in the moss Physcomitrella patens
Authors:
Arif, Muhammad Asif; Frank, Wolfgang; Khraiwesh, Basel
Abstract:
RNA interference (RNAi) is a mechanism that regulates genes by either transcriptional (TGS) or posttranscriptional gene silencing (PTGS), required for genome maintenance and proper development of an organism. Small non-coding RNAs are the key players in RNAi and have been intensively studied in eukaryotes. In plants, several classes of small RNAs with specific sizes and dedicated functions have evolved. The major classes of small RNAs include microRNAs (miRNAs) and small interfering RNAs (siRNAs), which differ in their biogenesis. miRNAs are synthesized from a short hairpin structure while siRNAs are derived from long double-stranded RNAs (dsRNA). Both miRNA and siRNAs control the expression of cognate target RNAs by binding to reverse complementary sequences mediating cleavage or translational inhibition of the target RNA. They also act on the DNA and cause epigenetic changes such as DNA methylation and histone modifications. In the last years, the analysis of plant RNAi pathways was extended to the bryophyte Physcomitrella patens, a non-flowering, non-vascular ancient land plant that diverged from the lineage of seed plants approximately 450 million years ago. Based on a number of characteristic features and its phylogenetic key position in land plant evolution P. patens emerged as a plant model species to address basic as well as applied topics in plant biology. Here we summarize the current knowledge on the role of RNAi in P. patens that shows functional overlap with RNAi pathways from seed plants, and also unique features specific to this species. 2013 by the authors; licensee MDPI, Basel, Switzerland.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture
Citation:
Arif M, Frank W, Khraiwesh B (2013) Role of RNA Interference (RNAi) in the Moss Physcomitrella patens. International Journal of Molecular Sciences 14: 1516-1540. doi:10.3390/ijms14011516.
Publisher:
MDPI
Journal:
International Journal of Molecular Sciences
Issue Date:
14-Jan-2013
DOI:
10.3390/ijms14011516
PubMed ID:
23344055
PubMed Central ID:
PMC3565333
Type:
Article
ISSN:
16616596
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorArif, Muhammad Asifen
dc.contributor.authorFrank, Wolfgangen
dc.contributor.authorKhraiwesh, Baselen
dc.date.accessioned2014-08-27T09:48:51Z-
dc.date.available2014-08-27T09:48:51Z-
dc.date.issued2013-01-14en
dc.identifier.citationArif M, Frank W, Khraiwesh B (2013) Role of RNA Interference (RNAi) in the Moss Physcomitrella patens. International Journal of Molecular Sciences 14: 1516-1540. doi:10.3390/ijms14011516.en
dc.identifier.issn16616596en
dc.identifier.pmid23344055en
dc.identifier.doi10.3390/ijms14011516en
dc.identifier.urihttp://hdl.handle.net/10754/325355en
dc.description.abstractRNA interference (RNAi) is a mechanism that regulates genes by either transcriptional (TGS) or posttranscriptional gene silencing (PTGS), required for genome maintenance and proper development of an organism. Small non-coding RNAs are the key players in RNAi and have been intensively studied in eukaryotes. In plants, several classes of small RNAs with specific sizes and dedicated functions have evolved. The major classes of small RNAs include microRNAs (miRNAs) and small interfering RNAs (siRNAs), which differ in their biogenesis. miRNAs are synthesized from a short hairpin structure while siRNAs are derived from long double-stranded RNAs (dsRNA). Both miRNA and siRNAs control the expression of cognate target RNAs by binding to reverse complementary sequences mediating cleavage or translational inhibition of the target RNA. They also act on the DNA and cause epigenetic changes such as DNA methylation and histone modifications. In the last years, the analysis of plant RNAi pathways was extended to the bryophyte Physcomitrella patens, a non-flowering, non-vascular ancient land plant that diverged from the lineage of seed plants approximately 450 million years ago. Based on a number of characteristic features and its phylogenetic key position in land plant evolution P. patens emerged as a plant model species to address basic as well as applied topics in plant biology. Here we summarize the current knowledge on the role of RNAi in P. patens that shows functional overlap with RNAi pathways from seed plants, and also unique features specific to this species. 2013 by the authors; licensee MDPI, Basel, Switzerland.en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0en
dc.subjectGene silencingen
dc.subjectMiRNAen
dc.subjectNon-coding RNAsen
dc.subjectPhyscomitrella patensen
dc.subjectRNAien
dc.subjectsiRNAen
dc.subjectcomplementary RNAen
dc.subjectdouble stranded RNAen
dc.subjectmicroRNAen
dc.subjectplant RNAen
dc.subjectshort hairpin RNAen
dc.subjectsmall interfering RNAen
dc.subjectsmall untranslated RNAen
dc.subjectvegetable proteinen
dc.subjectDNA methylationen
dc.subjectepigeneticsen
dc.subjecteukaryoteen
dc.subjectgene controlen
dc.subjectgene expression regulationen
dc.subjecthistone modificationen
dc.subjectmolecular evolutionen
dc.subjectnucleotide sequenceen
dc.subjectphylogenyen
dc.subjectplant geneen
dc.subjectplant genomeen
dc.subjectplant seeden
dc.subjectposttranscriptional gene silencingen
dc.subjectreviewen
dc.subjectRNA bindingen
dc.subjectRNA interferenceen
dc.subjectRNA sequenceen
dc.subjectRNA structureen
dc.subjectRNA synthesisen
dc.subjectsequence homologyen
dc.subjecttranscription regulationen
dc.subjecttranslation regulationen
dc.subjectBryophytaen
dc.subjectbryophytesen
dc.subjectEmbryophytaen
dc.subjectEukaryotaen
dc.subjectSpermatophytaen
dc.titleRole of RNA interference (RNAi) in the moss Physcomitrella patensen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalInternational Journal of Molecular Sciencesen
dc.identifier.pmcidPMC3565333en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Molecular Developmental Biology, Nijmegen Center for Molecular Life Sciences, 6500 HB Nijmegen, Netherlandsen
dc.contributor.institutionPlant Molecular Cell Biology, Department I, Ludwig-Maximilians-University Munich, LMU Biocenter, Grosshaderner Strasse 2-4, 82152 Planegg-Martinsried, Germanyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKhraiwesh, Baselen

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