Diversity and distribution of eukaryotic microbes in and around a brine pool adjacent to the Thuwal cold seeps in the Red Sea

Handle URI:
http://hdl.handle.net/10754/325351
Title:
Diversity and distribution of eukaryotic microbes in and around a brine pool adjacent to the Thuwal cold seeps in the Red Sea
Authors:
Wang, Yong; Zhang, Wei Peng; Cao, Hui Luo; Shek, Chun Shum; Tian, Ren Mao; Wong, Yue Him; Batang, Zenon B. ( 0000-0003-4593-0589 ) ; Al-Suwailem, Abdulaziz M.; Qian, Pei-Yuan
Abstract:
A hypoxic/suboxic brine pool at a depth of about 850 m was discovered near the Thuwal cold seeps in the Red Sea. Filled with high concentrations of hydrogen sulfide and ammonia, such a brine pool might limit the spread of eukaryotic organisms. Here, we compared the communities of the eukaryotic microbes in a microbial mat, sediments and water samples distributed in 7 sites within and adjacent to the brine pool. Taxonomic classification of the pyrosequenced 18S rRNA amplicon reads showed that fungi highly similar to the species identified along the Arabic coast were almost ubiquitous in the water and sediment samples, supporting their wide distribution in various environments. The microbial mat displayed the highest species diversity and contained grazers and a considerable percentage of unclassified species. Phylogeny-based methods revealed novel lineages representing a majority of the reads from the interface between the sea water and brine pool. Phylogenetic relationships with more reference sequences suggest that the lineages were affiliated with novel Alveolata and Euglenozoa inhabiting the interface where chemosynthetic prokaryotes are highly proliferative due to the strong chemocline and halocline. The brine sediments harbored abundant species highly similar to invertebrate gregarine parasites identified in different oxygen-depleted sediments. Therefore, the present findings support the uniqueness of some microbial eukaryotic groups in this cold seep brine system. 2014 Wang, Zhang, Cao, Shek, Tian, Wong, Batang, Al-suwailem and Qian.
KAUST Department:
Coastal and Marine Resources Core Lab
Citation:
Wang Y, Zhang WP, Cao HL, Shek CS, Tian RM, et al. (2014) Diversity and distribution of eukaryotic microbes in and around a brine pool adjacent to the Thuwal cold seeps in the Red Sea. Frontiers in Microbiology 5. doi:10.3389/fmicb.2014.00037.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
Issue Date:
4-Feb-2014
DOI:
10.3389/fmicb.2014.00037
PubMed ID:
24575081
PubMed Central ID:
PMC3922051
Type:
Article
ISSN:
1664302X
Appears in Collections:
Articles; Coastal and Marine Resources Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Yongen
dc.contributor.authorZhang, Wei Pengen
dc.contributor.authorCao, Hui Luoen
dc.contributor.authorShek, Chun Shumen
dc.contributor.authorTian, Ren Maoen
dc.contributor.authorWong, Yue Himen
dc.contributor.authorBatang, Zenon B.en
dc.contributor.authorAl-Suwailem, Abdulaziz M.en
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-08-27T09:48:42Z-
dc.date.available2014-08-27T09:48:42Z-
dc.date.issued2014-02-04en
dc.identifier.citationWang Y, Zhang WP, Cao HL, Shek CS, Tian RM, et al. (2014) Diversity and distribution of eukaryotic microbes in and around a brine pool adjacent to the Thuwal cold seeps in the Red Sea. Frontiers in Microbiology 5. doi:10.3389/fmicb.2014.00037.en
dc.identifier.issn1664302Xen
dc.identifier.pmid24575081en
dc.identifier.doi10.3389/fmicb.2014.00037en
dc.identifier.urihttp://hdl.handle.net/10754/325351en
dc.description.abstractA hypoxic/suboxic brine pool at a depth of about 850 m was discovered near the Thuwal cold seeps in the Red Sea. Filled with high concentrations of hydrogen sulfide and ammonia, such a brine pool might limit the spread of eukaryotic organisms. Here, we compared the communities of the eukaryotic microbes in a microbial mat, sediments and water samples distributed in 7 sites within and adjacent to the brine pool. Taxonomic classification of the pyrosequenced 18S rRNA amplicon reads showed that fungi highly similar to the species identified along the Arabic coast were almost ubiquitous in the water and sediment samples, supporting their wide distribution in various environments. The microbial mat displayed the highest species diversity and contained grazers and a considerable percentage of unclassified species. Phylogeny-based methods revealed novel lineages representing a majority of the reads from the interface between the sea water and brine pool. Phylogenetic relationships with more reference sequences suggest that the lineages were affiliated with novel Alveolata and Euglenozoa inhabiting the interface where chemosynthetic prokaryotes are highly proliferative due to the strong chemocline and halocline. The brine sediments harbored abundant species highly similar to invertebrate gregarine parasites identified in different oxygen-depleted sediments. Therefore, the present findings support the uniqueness of some microbial eukaryotic groups in this cold seep brine system. 2014 Wang, Zhang, Cao, Shek, Tian, Wong, Batang, Al-suwailem and Qian.en
dc.language.isoenen
dc.publisherFrontiers Media SAen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subject18S rRNAen
dc.subjectBrine poolen
dc.subjectClassificationen
dc.subjectCold seepen
dc.subjectEukaryotic microbesen
dc.subjectammoniaen
dc.subjectnitrogenen
dc.subjectRNA 18Sen
dc.subjectcold seep speciesen
dc.subjectDNA extractionen
dc.subjectDNA isolationen
dc.subjectmicrobial maten
dc.subjectnucleotide sequenceen
dc.subjectphylogenyen
dc.subjectpolymerase chain reactionen
dc.subjectprincipal component analysisen
dc.subjectpyrosequencingen
dc.subjectsedimenten
dc.subjectspecies diversityen
dc.subjecttotal organic carbonen
dc.subjectultraviolet spectrophotometryen
dc.subjectwater samplingen
dc.titleDiversity and distribution of eukaryotic microbes in and around a brine pool adjacent to the Thuwal cold seeps in the Red Seaen
dc.typeArticleen
dc.contributor.departmentCoastal and Marine Resources Core Laben
dc.identifier.journalFrontiers in Microbiologyen
dc.identifier.pmcidPMC3922051en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Life Science, Hong Kong University of Science and Technology, Hong Kongen
dc.contributor.institutionSanya Institute of Deep Sea Science and Engineering, Chinese Academy of Sciences, San Ya, Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAl-Suwailem, Abdulaziz M.en
kaust.authorBatang, Zenon B.en

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