High-throughput phenotyping to detect drought tolerance QTL in wild barley introgression lines

Handle URI:
http://hdl.handle.net/10754/325338
Title:
High-throughput phenotyping to detect drought tolerance QTL in wild barley introgression lines
Authors:
Honsdorf, Nora; March, Timothy John; Berger, Bettina; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Pillen, Klaus
Abstract:
Drought is one of the most severe stresses, endangering crop yields worldwide. In order to select drought tolerant genotypes, access to exotic germplasm and efficient phenotyping protocols are needed. In this study the high-throughput phenotyping platform "The Plant Accelerator", Adelaide, Australia, was used to screen a set of 47 juvenile (six week old) wild barley introgression lines (S42ILs) for drought stress responses. The kinetics of growth development was evaluated under early drought stress and well watered treatments. High correlation (r = 0.98) between image based biomass estimates and actual biomass was demonstrated, and the suitability of the system to accurately and non-destructively estimate biomass was validated. Subsequently, quantitative trait loci (QTL) were located, which contributed to the genetic control of growth under drought stress. In total, 44 QTL for eleven out of 14 investigated traits were mapped, which for example controlled growth rate and water use efficiency. The correspondence of those QTL with QTL previously identified in field trials is shown. For instance, six out of eight QTL controlling plant height were also found in previous field and glasshouse studies with the same introgression lines. This indicates that phenotyping juvenile plants may assist in predicting adult plant performance. In addition, favorable wild barley alleles for growth and biomass parameters were detected, for instance, a QTL that increased biomass by approximately 36%. In particular, introgression line S42IL-121 revealed improved growth under drought stress compared to the control Scarlett. The introgression line showed a similar behavior in previous field experiments, indicating that S42IL-121 may be an attractive donor for breeding of drought tolerant barley cultivars. © 2014 Honsdorf et al.
KAUST Department:
Center for Desert Agriculture
Citation:
Honsdorf N, March TJ, Berger B, Tester M, Pillen K (2014) High-Throughput Phenotyping to Detect Drought Tolerance QTL in Wild Barley Introgression Lines. PLoS ONE 9: e97047. doi:10.1371/journal.pone.0097047.
Publisher:
Public Library of Science
Journal:
PLoS ONE
Issue Date:
13-May-2014
DOI:
10.1371/journal.pone.0097047
PubMed ID:
24823485
PubMed Central ID:
PMC4019662
Type:
Article
ISSN:
19326203
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorHonsdorf, Noraen
dc.contributor.authorMarch, Timothy Johnen
dc.contributor.authorBerger, Bettinaen
dc.contributor.authorTester, Mark A.en
dc.contributor.authorPillen, Klausen
dc.date.accessioned2014-08-27T09:47:47Zen
dc.date.available2014-08-27T09:47:47Zen
dc.date.issued2014-05-13en
dc.identifier.citationHonsdorf N, March TJ, Berger B, Tester M, Pillen K (2014) High-Throughput Phenotyping to Detect Drought Tolerance QTL in Wild Barley Introgression Lines. PLoS ONE 9: e97047. doi:10.1371/journal.pone.0097047.en
dc.identifier.issn19326203en
dc.identifier.pmid24823485en
dc.identifier.doi10.1371/journal.pone.0097047en
dc.identifier.urihttp://hdl.handle.net/10754/325338en
dc.description.abstractDrought is one of the most severe stresses, endangering crop yields worldwide. In order to select drought tolerant genotypes, access to exotic germplasm and efficient phenotyping protocols are needed. In this study the high-throughput phenotyping platform "The Plant Accelerator", Adelaide, Australia, was used to screen a set of 47 juvenile (six week old) wild barley introgression lines (S42ILs) for drought stress responses. The kinetics of growth development was evaluated under early drought stress and well watered treatments. High correlation (r = 0.98) between image based biomass estimates and actual biomass was demonstrated, and the suitability of the system to accurately and non-destructively estimate biomass was validated. Subsequently, quantitative trait loci (QTL) were located, which contributed to the genetic control of growth under drought stress. In total, 44 QTL for eleven out of 14 investigated traits were mapped, which for example controlled growth rate and water use efficiency. The correspondence of those QTL with QTL previously identified in field trials is shown. For instance, six out of eight QTL controlling plant height were also found in previous field and glasshouse studies with the same introgression lines. This indicates that phenotyping juvenile plants may assist in predicting adult plant performance. In addition, favorable wild barley alleles for growth and biomass parameters were detected, for instance, a QTL that increased biomass by approximately 36%. In particular, introgression line S42IL-121 revealed improved growth under drought stress compared to the control Scarlett. The introgression line showed a similar behavior in previous field experiments, indicating that S42IL-121 may be an attractive donor for breeding of drought tolerant barley cultivars. © 2014 Honsdorf et al.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectalleleen
dc.subjectbarleyen
dc.subjectbiomass productionen
dc.subjectcontrolled studyen
dc.subjectcultivaren
dc.subjectdrought toleranceen
dc.subjectgene identificationen
dc.subjectgenetic analyzeren
dc.subjectgenetic lineen
dc.subjectgenetic regulationen
dc.subjectgrowth curveen
dc.subjecthigh throughput phenotyping platformen
dc.subjectintrogressionen
dc.subjectjuvenile planten
dc.subjectphenotypeen
dc.subjectplant growthen
dc.subjectplant heighten
dc.subjectplant water useen
dc.subjectquantitative trait locusen
dc.subjectquantitative trait locus mappingen
dc.subjectvalidation processen
dc.subjectwild speciesen
dc.titleHigh-throughput phenotyping to detect drought tolerance QTL in wild barley introgression linesen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC4019662en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Plant Breeding, Institute of Agricultural and Nutritional Sciences, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germanyen
dc.contributor.institutionInterdisciplinary Center for Crop Plant Research (IZN), Halle (Saale), Germanyen
dc.contributor.institutionSchool of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Adelaide, SA, Australiaen
dc.contributor.institutionPlant Accelerator, University of Adelaide, Waite Campus, Adelaide, SA, Australiaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorTester, Mark A.en
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