Quantitative proteomics study of larval settlement in the barnacle Balanus amphitrite

Handle URI:
http://hdl.handle.net/10754/325336
Title:
Quantitative proteomics study of larval settlement in the barnacle Balanus amphitrite
Authors:
Chen, Zhang-Fan; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Wang, Hao; Matsumura, Kiyotaka; Wong, Yue Him; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Qian, Pei-Yuan
Abstract:
Barnacles are major sessile components of the intertidal areas worldwide, and also one of the most dominant fouling organisms in fouling communities. Larval settlement has a crucial ecological effect not only on the distribution of the barnacle population but also intertidal community structures. However, the molecular mechanisms involved in the transition process from the larval to the juvenile stage remain largely unclear. In this study, we carried out comparative proteomic profiles of stage II nauplii, stage VI nauplii, cyprids, and juveniles of the barnacle Balanus amphitrite using label-free quantitative proteomics, followed by the measurement of the gene expression levels of candidate proteins. More than 700 proteins were identified at each stage; 80 were significantly up-regulated in cyprids and 95 in juveniles vs other stages. Specifically, proteins involved in energy and metabolism, the nervous system and signal transduction were significantly up-regulated in cyprids, whereas proteins involved in cytoskeletal remodeling, transcription and translation, cell proliferation and differentiation, and biomineralization were up-regulated in juveniles, consistent with changes associated with larval metamorphosis and tissue remodeling in juveniles. These findings provided molecular evidence for the morphological, physiological and biological changes that occur during the transition process from the larval to the juvenile stages in B. amphitrite. © 2014 Chen et al.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; KAUST Global Collaborative Research Program; Biosciences Core Lab
Citation:
Chen Z-F, Zhang H, Wang H, Matsumura K, Wong YH, et al. (2014) Quantitative Proteomics Study of Larval Settlement in the Barnacle Balanus amphitrite. PLoS ONE 9: e88744. doi:10.1371/journal.pone.0088744.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
13-Feb-2014
DOI:
10.1371/journal.pone.0088744
PubMed ID:
24551147
PubMed Central ID:
PMC3923807
Type:
Article
ISSN:
19326203
Appears in Collections:
Articles; Biosciences Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Zhang-Fanen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorWang, Haoen
dc.contributor.authorMatsumura, Kiyotakaen
dc.contributor.authorWong, Yue Himen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-08-27T09:47:40Zen
dc.date.available2014-08-27T09:47:40Zen
dc.date.issued2014-02-13en
dc.identifier.citationChen Z-F, Zhang H, Wang H, Matsumura K, Wong YH, et al. (2014) Quantitative Proteomics Study of Larval Settlement in the Barnacle Balanus amphitrite. PLoS ONE 9: e88744. doi:10.1371/journal.pone.0088744.en
dc.identifier.issn19326203en
dc.identifier.pmid24551147en
dc.identifier.doi10.1371/journal.pone.0088744en
dc.identifier.urihttp://hdl.handle.net/10754/325336en
dc.description.abstractBarnacles are major sessile components of the intertidal areas worldwide, and also one of the most dominant fouling organisms in fouling communities. Larval settlement has a crucial ecological effect not only on the distribution of the barnacle population but also intertidal community structures. However, the molecular mechanisms involved in the transition process from the larval to the juvenile stage remain largely unclear. In this study, we carried out comparative proteomic profiles of stage II nauplii, stage VI nauplii, cyprids, and juveniles of the barnacle Balanus amphitrite using label-free quantitative proteomics, followed by the measurement of the gene expression levels of candidate proteins. More than 700 proteins were identified at each stage; 80 were significantly up-regulated in cyprids and 95 in juveniles vs other stages. Specifically, proteins involved in energy and metabolism, the nervous system and signal transduction were significantly up-regulated in cyprids, whereas proteins involved in cytoskeletal remodeling, transcription and translation, cell proliferation and differentiation, and biomineralization were up-regulated in juveniles, consistent with changes associated with larval metamorphosis and tissue remodeling in juveniles. These findings provided molecular evidence for the morphological, physiological and biological changes that occur during the transition process from the larval to the juvenile stages in B. amphitrite. © 2014 Chen et al.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectvitellogeninen
dc.subjectAmphibalanus amphitriteen
dc.subjectbiomineralizationen
dc.subjectcell differentiationen
dc.subjectcell proliferationen
dc.subjectcomparative studyen
dc.subjectcypris (larva)en
dc.subjectcytoskeletonen
dc.subjectenergyen
dc.subjectgene expressionen
dc.subjectgenetic transcription and translationen
dc.subjectjuvenile animalen
dc.subjectlarvaen
dc.subjectliquid chromatographyen
dc.subjectmass spectrometryen
dc.subjectmetabolismen
dc.subjectmetamorphosisen
dc.subjectnervous systemen
dc.subjectnucleotide sequenceen
dc.subjectphylogenyen
dc.subjectprotein analysisen
dc.subjectproteomicsen
dc.subjectquantitative analysisen
dc.subjectreal time polymerase chain reactionen
dc.subjectsignal transductionen
dc.subjectupregulationen
dc.titleQuantitative proteomics study of larval settlement in the barnacle Balanus amphitriteen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3923807en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionUnidad Académica de Sistemas Arrecifales (Puerto Morelos), Instituto de Ciencias Del Mar y Limnología, Universidad Nacional Autõnoma de México, Puerto Morelos, QR 77580, Mexicoen
dc.contributor.institutionSchool of Natural Sciences, University of California Merced, 5200 North Lake Road, Merced, CA 95343, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorZhang, Huomingen
kaust.authorRavasi, Timothyen
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