Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning

Handle URI:
http://hdl.handle.net/10754/325325
Title:
Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning
Authors:
Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Reish, Donald; Qian, Pei-Yuan
Abstract:
The Neanthes acuminata species complex (Polychaeta) are cosmopolitan in distribution. Neanthes arenaceodentata, complex, has been widely used as toxicological test animal in the marine environment. Method of reproduction is unique in this polychaete complex. Same sexes fight and opposite sexes lie side by side until egg laying. Females lose about 75% of their weight and die after laying eggs. The male, capable of reproducing up to nine times, fertilizes the eggs and incubates the embryos for 3-4 weeks. The objective of this study was to determine if there is any set of proteins that influences this unique pattern of reproduction. Gel-based two-dimensional electrophoresis (2-DE) and gel-free quantitative proteomics methods were used to identify differential protein expression patterns before and after spawning in both male and female N. arenaceodentata. Males showed a higher degree of similarity in protein expression patterns but females showed large changes in phosphoproteme before and after spawning. There was a decrease (about 70%) in the number of detected phosphoproteins in spent females. The proteins involved in muscular development, cell signaling, structure and integrity, and translation were differentially expressed. This study provides proteomic insights of the male and female worms that may serve as a foundation for better understanding of unusual reproductive patterns in polychaete worms. © 2013 Chandramouli et al.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; KAUST Global Collaborative Research Program
Citation:
Chandramouli KH, Ravasi T, Reish D, Qian P-Y (2013) Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning. PLoS ONE 8: e72990. doi:10.1371/journal.pone.0072990.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
30-Aug-2013
DOI:
10.1371/journal.pone.0072990
PubMed ID:
24023665
PubMed Central ID:
PMC3758283
Type:
Article
ISSN:
19326203
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorReish, Donalden
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-08-27T09:47:03Zen
dc.date.available2014-08-27T09:47:03Zen
dc.date.issued2013-08-30en
dc.identifier.citationChandramouli KH, Ravasi T, Reish D, Qian P-Y (2013) Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning. PLoS ONE 8: e72990. doi:10.1371/journal.pone.0072990.en
dc.identifier.issn19326203en
dc.identifier.pmid24023665en
dc.identifier.doi10.1371/journal.pone.0072990en
dc.identifier.urihttp://hdl.handle.net/10754/325325en
dc.description.abstractThe Neanthes acuminata species complex (Polychaeta) are cosmopolitan in distribution. Neanthes arenaceodentata, complex, has been widely used as toxicological test animal in the marine environment. Method of reproduction is unique in this polychaete complex. Same sexes fight and opposite sexes lie side by side until egg laying. Females lose about 75% of their weight and die after laying eggs. The male, capable of reproducing up to nine times, fertilizes the eggs and incubates the embryos for 3-4 weeks. The objective of this study was to determine if there is any set of proteins that influences this unique pattern of reproduction. Gel-based two-dimensional electrophoresis (2-DE) and gel-free quantitative proteomics methods were used to identify differential protein expression patterns before and after spawning in both male and female N. arenaceodentata. Males showed a higher degree of similarity in protein expression patterns but females showed large changes in phosphoproteme before and after spawning. There was a decrease (about 70%) in the number of detected phosphoproteins in spent females. The proteins involved in muscular development, cell signaling, structure and integrity, and translation were differentially expressed. This study provides proteomic insights of the male and female worms that may serve as a foundation for better understanding of unusual reproductive patterns in polychaete worms. © 2013 Chandramouli et al.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS ONEen
dc.subjectphosphoproteinen
dc.subjectcontrolled studyen
dc.subjectegg layingen
dc.subjectfertilizationen
dc.subjectimage analysisen
dc.subjectmass spectrometryen
dc.subjectmuscle developmenten
dc.subjectNeanthes arenaceodentataen
dc.subjectnucleotide sequenceen
dc.subjectPolychaetaen
dc.subjectprotein expressionen
dc.subjectprotein phosphorylationen
dc.subjectproteomicsen
dc.subjectsignal transductionen
dc.subjectspawningen
dc.subjecttwo dimensional electrophoresisen
dc.subjectActinsen
dc.subjectBlotting, Westernen
dc.subjectElectrophoresis, Gel, Two-Dimensionalen
dc.subjectPhosphoproteinsen
dc.subjectPhosphorylationen
dc.subjectPolychaetaen
dc.subjectProtein Isoformsen
dc.subjectProteomeen
dc.subjectProteomicsen
dc.subjectReproducibility of Resultsen
dc.subjectReproductionen
dc.titleProteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawningen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3758283en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionIntegrative Systems Biology Laboratory, Division of Biological and Environmental Sciences and Engineering, Div. of Applied Mathematics and Computer Sciences, King Abdullah University of Sci. and Technology, Thuwal, Saudi Arabiaen
dc.contributor.institutionDepartment of Biological Sciences, California State University Long Beach, California, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorRavasi, Timothyen
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