Involvement of wnt signaling pathways in the metamorphosis of the bryozoan bugula neritina

Handle URI:
http://hdl.handle.net/10754/325301
Title:
Involvement of wnt signaling pathways in the metamorphosis of the bryozoan bugula neritina
Authors:
Wong, Yue Him; Wang, Hao; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Qian, Pei-Yuan
Abstract:
In this study, we analyzed the metamorphosis of the marine bryozoan Bugula neritina. We observed the morphogenesis of the ancestrula. We defined three distinct pre-ancestrula stages based on the anatomy of the developing polypide and the overall morphology of pre-ancestrula. We then used an annotation based enrichment analysis tool to analyze the B. neritina transcriptome and identified over-representation of genes related to Wnt signaling pathways, suggesting its involvement in metamorphosis. Finally, we studied the temporal-spatial gene expression studies of several Wnt pathway genes. We found that one of the Wnt ligand, BnWnt10, was expressed spatially opposite to the Wnt antagonist BnsFRP within the blastemas, which is the presumptive polypide. Down-stream components of the canonical Wnt signaling pathway were exclusively expressed in the blastemas. Bn?catenin and BnFz5/8 were exclusively expressed in the blastemas throughout the metamorphosis. Based on the genes expression patterns, we propose that BnWnt10 and BnsFRP may relate to the patterning of the polypide, in which the two genes served as positional signals and contributed to the polarization of the blastemas. Another Wnt ligand, BnWnt6, was expressed in the apical part of the pre-ancestrula epidermis. Overall, our findings suggest that the Wnt signaling pathway may be important to the pattern formation of polypide and the development of epidermis. © 2012 Wong et al.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; KAUST Global Collaborative Research Program; Computational Bioscience Research Center (CBRC)
Citation:
Wong YH, Wang H, Ravasi T, Qian P-Y (2012) Involvement of Wnt Signaling Pathways in the Metamorphosis of the Bryozoan Bugula neritina. PLoS ONE 7: e33323. doi:10.1371/journal.pone.0033323.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
20-Mar-2012
DOI:
10.1371/journal.pone.0033323
PubMed ID:
22448242
PubMed Central ID:
PMC3308966
Type:
Article
ISSN:
19326203
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWong, Yue Himen
dc.contributor.authorWang, Haoen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-08-27T09:45:51Z-
dc.date.available2014-08-27T09:45:51Z-
dc.date.issued2012-03-20en
dc.identifier.citationWong YH, Wang H, Ravasi T, Qian P-Y (2012) Involvement of Wnt Signaling Pathways in the Metamorphosis of the Bryozoan Bugula neritina. PLoS ONE 7: e33323. doi:10.1371/journal.pone.0033323.en
dc.identifier.issn19326203en
dc.identifier.pmid22448242en
dc.identifier.doi10.1371/journal.pone.0033323en
dc.identifier.urihttp://hdl.handle.net/10754/325301en
dc.description.abstractIn this study, we analyzed the metamorphosis of the marine bryozoan Bugula neritina. We observed the morphogenesis of the ancestrula. We defined three distinct pre-ancestrula stages based on the anatomy of the developing polypide and the overall morphology of pre-ancestrula. We then used an annotation based enrichment analysis tool to analyze the B. neritina transcriptome and identified over-representation of genes related to Wnt signaling pathways, suggesting its involvement in metamorphosis. Finally, we studied the temporal-spatial gene expression studies of several Wnt pathway genes. We found that one of the Wnt ligand, BnWnt10, was expressed spatially opposite to the Wnt antagonist BnsFRP within the blastemas, which is the presumptive polypide. Down-stream components of the canonical Wnt signaling pathway were exclusively expressed in the blastemas. Bn?catenin and BnFz5/8 were exclusively expressed in the blastemas throughout the metamorphosis. Based on the genes expression patterns, we propose that BnWnt10 and BnsFRP may relate to the patterning of the polypide, in which the two genes served as positional signals and contributed to the polarization of the blastemas. Another Wnt ligand, BnWnt6, was expressed in the apical part of the pre-ancestrula epidermis. Overall, our findings suggest that the Wnt signaling pathway may be important to the pattern formation of polypide and the development of epidermis. © 2012 Wong et al.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsWong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS ONEen
dc.subjecttranscriptomeen
dc.subjectbiological markeren
dc.subjectmessenger RNAen
dc.subjecttranscriptomeen
dc.subjectanimal tissueen
dc.subjectbeta catenin geneen
dc.subjectblastemaen
dc.subjectBryozoaen
dc.subjectBugula neritinaen
dc.subjectcontrolled studyen
dc.subjectdevelopmental geneen
dc.subjectdevelopmental stageen
dc.subjectembryo pattern formationen
dc.subjectFz5 8 geneen
dc.subjectgene expression profilingen
dc.subjectgene functionen
dc.subjectgene identificationen
dc.subjectgene locationen
dc.subjectmetamorphosisen
dc.subjectmorphogenesisen
dc.subjectWnt signaling pathwayen
dc.subjectWnt10 geneen
dc.subjectBryozoaen
dc.subjectDNA microarrayen
dc.subjectgene expression regulationen
dc.subjectgeneticsen
dc.subjectgrowth, development and agingen
dc.subjectin situ hybridizationen
dc.subjectlarvaen
dc.subjectmetabolismen
dc.subjectreal time polymerase chain reactionen
dc.subjectreverse transcription polymerase chain reactionen
dc.subjectsignal transductionen
dc.subjectBryozoaen
dc.subjectBugula neritinaen
dc.subjectGymnolaemataen
dc.subjectBiological Markersen
dc.subjectBryozoaen
dc.subjectGene Expression Regulation, Developmentalen
dc.subjectIn Situ Hybridizationen
dc.subjectLarvaen
dc.subjectMetamorphosis, Biologicalen
dc.subjectOligonucleotide Array Sequence Analysisen
dc.subjectReal-Time Polymerase Chain Reactionen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectRNA, Messengeren
dc.subjectSignal Transductionen
dc.subjectTranscriptomeen
dc.subjectWnt Signaling Pathwayen
dc.titleInvolvement of wnt signaling pathways in the metamorphosis of the bryozoan bugula neritinaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3308966en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionUnidad Académica de Sistemas Arrecifales (Puerto Morelos), Instituto de Ciencias Del Mar y Limnología, Universidad Nacional Autõnoma de México, Puerto Morelos, QR 77580, Mexicoen
dc.contributor.institutionSchool of Natural Sciences, University of California Merced, 5200 North Lake Road, Merced, CA 95343, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorRavasi, Timothyen
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