Mutation in SUMO E3 ligase, SIZ1, disrupts the mature female gametophyte in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/325299
Title:
Mutation in SUMO E3 ligase, SIZ1, disrupts the mature female gametophyte in Arabidopsis
Authors:
Ling, Yu ( 0000-0002-8067-523X ) ; Zhang, Chunyu; Chen, Tong; Hao, Huaiqing; Liu, Peng; Bressan, Ray A.; Hasegawa, Paul M.; Jin, Jing Bo; Lin, Jinxing
Abstract:
Female gametophyte is the multicellular haploid structure that can produce embryo and endosperm after fertilization, which has become an attractive model system for investigating molecular mechanisms in nuclei migration, cell specification, cell-to-cell communication and many other processes. Previous reports found that the small ubiquitin-like modifier (SUMO) E3 ligase, SIZ1, participated in many processes depending on particular target substrates and suppression of salicylic acid (SA) accumulation. Here, we report that SIZ1 mediates the reproductive process. SIZ1 showed enhanced expression in female organs, but was not detected in the anther or pollen. A defect in the siz1-2 maternal source resulted in reduced seed-set regardless of high SA concentration within the plant. Moreover, aniline blue staining and scanning electron microscopy revealed that funicular and micropylar pollen tube guidance was arrested in siz1-2 plants. Some of the embryo sacs of ovules in siz1-2 were also disrupted quickly after stage FG7. There was no significant affects of the siz1-2 mutation on expression of genes involved in female gametophyte development- or pollen tube guidance in ovaries. Together, our results suggest that SIZ1 sustains the stability and normal function of the mature female gametophyte which is necessary for pollen tube guidance. © 2012 Ling et al.
KAUST Department:
Plant Stress Genomics Research Lab
Citation:
Ling Y, Zhang C, Chen T, Hao H, Liu P, et al. (2012) Mutation in SUMO E3 ligase, SIZ1, Disrupts the Mature Female Gametophyte in Arabidopsis. PLoS ONE 7: e29470. doi:10.1371/journal.pone.0029470.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
9-Jan-2012
DOI:
10.1371/journal.pone.0029470
PubMed ID:
22253727
PubMed Central ID:
PMC3253799
Type:
Article
ISSN:
19326203
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorLing, Yuen
dc.contributor.authorZhang, Chunyuen
dc.contributor.authorChen, Tongen
dc.contributor.authorHao, Huaiqingen
dc.contributor.authorLiu, Pengen
dc.contributor.authorBressan, Ray A.en
dc.contributor.authorHasegawa, Paul M.en
dc.contributor.authorJin, Jing Boen
dc.contributor.authorLin, Jinxingen
dc.date.accessioned2014-08-27T09:45:46Z-
dc.date.available2014-08-27T09:45:46Z-
dc.date.issued2012-01-09en
dc.identifier.citationLing Y, Zhang C, Chen T, Hao H, Liu P, et al. (2012) Mutation in SUMO E3 ligase, SIZ1, Disrupts the Mature Female Gametophyte in Arabidopsis. PLoS ONE 7: e29470. doi:10.1371/journal.pone.0029470.en
dc.identifier.issn19326203en
dc.identifier.pmid22253727en
dc.identifier.doi10.1371/journal.pone.0029470en
dc.identifier.urihttp://hdl.handle.net/10754/325299en
dc.description.abstractFemale gametophyte is the multicellular haploid structure that can produce embryo and endosperm after fertilization, which has become an attractive model system for investigating molecular mechanisms in nuclei migration, cell specification, cell-to-cell communication and many other processes. Previous reports found that the small ubiquitin-like modifier (SUMO) E3 ligase, SIZ1, participated in many processes depending on particular target substrates and suppression of salicylic acid (SA) accumulation. Here, we report that SIZ1 mediates the reproductive process. SIZ1 showed enhanced expression in female organs, but was not detected in the anther or pollen. A defect in the siz1-2 maternal source resulted in reduced seed-set regardless of high SA concentration within the plant. Moreover, aniline blue staining and scanning electron microscopy revealed that funicular and micropylar pollen tube guidance was arrested in siz1-2 plants. Some of the embryo sacs of ovules in siz1-2 were also disrupted quickly after stage FG7. There was no significant affects of the siz1-2 mutation on expression of genes involved in female gametophyte development- or pollen tube guidance in ovaries. Together, our results suggest that SIZ1 sustains the stability and normal function of the mature female gametophyte which is necessary for pollen tube guidance. © 2012 Ling et al.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsLing et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS ONEen
dc.subjectprotein SIZ1en
dc.subjectSUMO 3 proteinen
dc.subjectunclassified drugen
dc.subjectArabidopsis proteinen
dc.subjectgreen fluorescent proteinen
dc.subjectligaseen
dc.subjectSIZ1 protein, Arabidopsisen
dc.subjectubiquitin protein ligaseen
dc.subjectArabidopsisen
dc.subjectcell divisionen
dc.subjectcontrolled studyen
dc.subjectembryo developmenten
dc.subjectfloweringen
dc.subjectgametophyteen
dc.subjectgene disruptionen
dc.subjectgene mutationen
dc.subjectgerminationen
dc.subjecthomozygosityen
dc.subjectinnate immunityen
dc.subjectovuleen
dc.subjectphenotypeen
dc.subjectplant geneticsen
dc.subjectplant reproductionen
dc.subjectpollinationen
dc.subjectprotein analysisen
dc.subjectprotein expressionen
dc.subjectquantitative analysisen
dc.subjectscanning electron microscopyen
dc.subjectsumoylationen
dc.subjectcytologyen
dc.subjectenzymologyen
dc.subjectgene expression regulationen
dc.subjectgeneticsen
dc.subjectgrowth, development and agingen
dc.subjecthomozygoteen
dc.subjectmetabolismen
dc.subjectmutationen
dc.subjectovuleen
dc.subjectplant gametogenesisen
dc.subjectplant geneen
dc.subjectplant seeden
dc.subjectpollen tubeen
dc.subjectprenatal developmenten
dc.subjectultrastructureen
dc.subjectArabidopsisen
dc.subjectArabidopsisen
dc.subjectArabidopsis Proteinsen
dc.subjectGametogenesis, Planten
dc.subjectGene Expression Regulation, Developmentalen
dc.subjectGene Expression Regulation, Planten
dc.subjectGenes, Planten
dc.subjectGreen Fluorescent Proteinsen
dc.subjectHomozygoteen
dc.subjectLigasesen
dc.subjectMutationen
dc.subjectOvuleen
dc.subjectPhenotypeen
dc.subjectPollen Tubeen
dc.subjectSeedsen
dc.subjectUbiquitin-Protein Ligasesen
dc.titleMutation in SUMO E3 ligase, SIZ1, disrupts the mature female gametophyte in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3253799en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, Chinaen
dc.contributor.institutionGraduate School of Chinese Academy of Sciences, Beijing, Chinaen
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, United Statesen
dc.contributor.institutionBiological Science Department, King Abdulaziz University, Jeddah, Saudi Arabiaen
dc.contributor.institutionDivision of Applied Life Sciences, Gyeongsang National University, Jinju, South Koreaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorBressan, Ray Anthonyen

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