Defence responses of arabidopsis thaliana to infection by pseudomonas syringae are regulated by the circadian clock

Handle URI:
http://hdl.handle.net/10754/325297
Title:
Defence responses of arabidopsis thaliana to infection by pseudomonas syringae are regulated by the circadian clock
Authors:
Bhardwaj, Vaibhav; Meier, Stuart; Petersen, Lindsay N.; Ingle, Robert A.; Roden, Laura C.
Abstract:
The circadian clock allows plants to anticipate predictable daily changes in abiotic stimuli, such as light; however, whether the clock similarly allows plants to anticipate interactions with other organisms is unknown. Here we show that Arabidopsis thaliana (Arabidopsis) has circadian clock-mediated variation in resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), with plants being least susceptible to infection in the subjective morning. We suggest that the increased resistance to Pst DC3000 observed in the morning in Col-0 plants results from clock-mediated modulation of pathogen associated molecular pattern (PAMP)-triggered immunity. Analysis of publicly available microarray data revealed that a large number of Arabidopsis defence-related genes showed both diurnal- and circadian-regulation, including genes involved in the perception of the PAMP flagellin which exhibit a peak in expression in the morning. Accordingly, we observed that PAMP-triggered callose deposition was significantly higher in wild-type plants inoculated with Pst DC3000 hrpA in the subjective morning than in the evening, while no such temporal difference was evident in arrhythmic plants. Our results suggest that PAMP-triggered immune responses are modulated by the circadian clock and that temporal regulation allows plants to anticipate and respond more effectively to pathogen challenges in the daytime. © 2011 Bhardwaj et al.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Bhardwaj V, Meier S, Petersen LN, Ingle RA, Roden LC (2011) Defence Responses of Arabidopsis thaliana to Infection by Pseudomonas syringae Are Regulated by the Circadian Clock. PLoS ONE 6: e26968. doi:10.1371/journal.pone.0026968.
Publisher:
Public Library of Science
Journal:
PLoS ONE
Issue Date:
31-Oct-2011
DOI:
10.1371/journal.pone.0026968
PubMed ID:
22066021
PubMed Central ID:
PMC3205005
Type:
Article
ISSN:
19326203
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBhardwaj, Vaibhaven
dc.contributor.authorMeier, Stuarten
dc.contributor.authorPetersen, Lindsay N.en
dc.contributor.authorIngle, Robert A.en
dc.contributor.authorRoden, Laura C.en
dc.date.accessioned2014-08-27T09:45:39Z-
dc.date.available2014-08-27T09:45:39Z-
dc.date.issued2011-10-31en
dc.identifier.citationBhardwaj V, Meier S, Petersen LN, Ingle RA, Roden LC (2011) Defence Responses of Arabidopsis thaliana to Infection by Pseudomonas syringae Are Regulated by the Circadian Clock. PLoS ONE 6: e26968. doi:10.1371/journal.pone.0026968.en
dc.identifier.issn19326203en
dc.identifier.pmid22066021en
dc.identifier.doi10.1371/journal.pone.0026968en
dc.identifier.urihttp://hdl.handle.net/10754/325297en
dc.description.abstractThe circadian clock allows plants to anticipate predictable daily changes in abiotic stimuli, such as light; however, whether the clock similarly allows plants to anticipate interactions with other organisms is unknown. Here we show that Arabidopsis thaliana (Arabidopsis) has circadian clock-mediated variation in resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), with plants being least susceptible to infection in the subjective morning. We suggest that the increased resistance to Pst DC3000 observed in the morning in Col-0 plants results from clock-mediated modulation of pathogen associated molecular pattern (PAMP)-triggered immunity. Analysis of publicly available microarray data revealed that a large number of Arabidopsis defence-related genes showed both diurnal- and circadian-regulation, including genes involved in the perception of the PAMP flagellin which exhibit a peak in expression in the morning. Accordingly, we observed that PAMP-triggered callose deposition was significantly higher in wild-type plants inoculated with Pst DC3000 hrpA in the subjective morning than in the evening, while no such temporal difference was evident in arrhythmic plants. Our results suggest that PAMP-triggered immune responses are modulated by the circadian clock and that temporal regulation allows plants to anticipate and respond more effectively to pathogen challenges in the daytime. © 2011 Bhardwaj et al.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rightsBhardwaj et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS ONEen
dc.subjectflagellinen
dc.subjectArabidopsis proteinen
dc.subjectcalloseen
dc.subjectglucanen
dc.subjectpattern recognition receptoren
dc.subjecttranscription factor CLOCKen
dc.subjectArabidopsisen
dc.subjectbacterial plant diseaseen
dc.subjectcircadian rhythmen
dc.subjectcontrolled studyen
dc.subjectgene expression profilingen
dc.subjectinfection resistanceen
dc.subjectinfection sensitivityen
dc.subjectplant defenseen
dc.subjectplant geneen
dc.subjectplant immunityen
dc.subjectPseudomonas syringaeen
dc.subjectcell wallen
dc.subjectdisease predispositionen
dc.subjectgene expression regulationen
dc.subjectgeneticsen
dc.subjectimmunologyen
dc.subjectlighten
dc.subjectmetabolismen
dc.subjectmicrobiologyen
dc.subjectmutationen
dc.subjectpathogenicityen
dc.subjectphysiologyen
dc.subjectplant diseaseen
dc.subjectradiation exposureen
dc.subjectsignal transductionen
dc.subjecttimeen
dc.subjectvirulenceen
dc.subjectArabidopsisen
dc.subjectArabidopsis thalianaen
dc.subjectBacteria (microorganisms)en
dc.subjectPseudomonas syringaeen
dc.subjectPseudomonas syringae pv. tomatoen
dc.subjectArabidopsisen
dc.subjectArabidopsis Proteinsen
dc.subjectCell Wallen
dc.subjectCircadian Clocksen
dc.subjectCircadian Rhythmen
dc.subjectCLOCK Proteinsen
dc.subjectDisease Susceptibilityen
dc.subjectGene Expression Profilingen
dc.subjectGene Expression Regulation, Planten
dc.subjectGenes, Planten
dc.subjectGlucansen
dc.subjectLighten
dc.subjectMutationen
dc.subjectPlant Diseasesen
dc.subjectPseudomonas syringaeen
dc.subjectReceptors, Pattern Recognitionen
dc.subjectSignal Transductionen
dc.subjectTime Factorsen
dc.subjectVirulenceen
dc.titleDefence responses of arabidopsis thaliana to infection by pseudomonas syringae are regulated by the circadian clocken
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3205005en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Molecular and Cell Biology, University of Cape Town, Rondebosch, South Africaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorMeier, Stuart Kurten
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