Toward an understanding of the molecular mechanisms of barnacle larval settlement: A comparative transcriptomic approach

Handle URI:
http://hdl.handle.net/10754/325292
Title:
Toward an understanding of the molecular mechanisms of barnacle larval settlement: A comparative transcriptomic approach
Authors:
Chen, Zhang-Fan; Matsumura, Kiyotaka; Wang, Hao; Arellano, Shawn M.; Yan, Xingcheng; Alam, Intikhab; Archer, John A.C. ( 0000-0002-3302-3933 ) ; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Qian, Pei-Yuan
Abstract:
Background: The barnacle Balanus amphitrite is a globally distributed biofouler and a model species in intertidal ecology and larval settlement studies. However, a lack of genomic information has hindered the comprehensive elucidation of the molecular mechanisms coordinating its larval settlement. The pyrosequencing-based transcriptomic approach is thought to be useful to identify key molecular changes during larval settlement. Methodology and Principal Findings: Using 454 pyrosequencing, we collected totally 630,845 reads including 215,308 from the larval stages and 415,537 from the adults; 23,451 contigs were generated while 77,785 remained as singletons. We annotated 31,720 of the 92,322 predicted open reading frames, which matched hits in the NCBI NR database, and identified 7,954 putative genes that were differentially expressed between the larval and adult stages. Of these, several genes were further characterized with quantitative real-time PCR and in situ hybridization, revealing some key findings: 1) vitellogenin was uniquely expressed in late nauplius stage, suggesting it may be an energy source for the subsequent non-feeding cyprid stage; 2) the locations of mannose receptors suggested they may be involved in the sensory system of cyprids; 3) 20 kDa-cement protein homologues were expressed in the cyprid cement gland and probably function during attachment; and 4) receptor tyrosine kinases were expressed higher in cyprid stage and may be involved in signal perception during larval settlement. Conclusions: Our results provide not only the basis of several new hypotheses about gene functions during larval settlement, but also the availability of this large transcriptome dataset in B. amphitrite for further exploration of larval settlement and developmental pathways in this important marine species. © 2011 Chen et al.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; KAUST Global Collaborative Research Program; Red Sea Research Center (RSRC)
Citation:
Chen Z-F, Matsumura K, Wang H, Arellano SM, Yan X, et al. (2011) Toward an Understanding of the Molecular Mechanisms of Barnacle Larval Settlement: A Comparative Transcriptomic Approach. PLoS ONE 6: e22913. doi:10.1371/journal.pone.0022913.
Publisher:
Public Library of Science
Journal:
PLoS ONE
Issue Date:
29-Jul-2011
DOI:
10.1371/journal.pone.0022913
PubMed ID:
21829555
PubMed Central ID:
PMC3146488
Type:
Article
ISSN:
19326203
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Zhang-Fanen
dc.contributor.authorMatsumura, Kiyotakaen
dc.contributor.authorWang, Haoen
dc.contributor.authorArellano, Shawn M.en
dc.contributor.authorYan, Xingchengen
dc.contributor.authorAlam, Intikhaben
dc.contributor.authorArcher, John A.C.en
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-08-27T09:45:19Z-
dc.date.available2014-08-27T09:45:19Z-
dc.date.issued2011-07-29en
dc.identifier.citationChen Z-F, Matsumura K, Wang H, Arellano SM, Yan X, et al. (2011) Toward an Understanding of the Molecular Mechanisms of Barnacle Larval Settlement: A Comparative Transcriptomic Approach. PLoS ONE 6: e22913. doi:10.1371/journal.pone.0022913.en
dc.identifier.issn19326203en
dc.identifier.pmid21829555en
dc.identifier.doi10.1371/journal.pone.0022913en
dc.identifier.urihttp://hdl.handle.net/10754/325292en
dc.description.abstractBackground: The barnacle Balanus amphitrite is a globally distributed biofouler and a model species in intertidal ecology and larval settlement studies. However, a lack of genomic information has hindered the comprehensive elucidation of the molecular mechanisms coordinating its larval settlement. The pyrosequencing-based transcriptomic approach is thought to be useful to identify key molecular changes during larval settlement. Methodology and Principal Findings: Using 454 pyrosequencing, we collected totally 630,845 reads including 215,308 from the larval stages and 415,537 from the adults; 23,451 contigs were generated while 77,785 remained as singletons. We annotated 31,720 of the 92,322 predicted open reading frames, which matched hits in the NCBI NR database, and identified 7,954 putative genes that were differentially expressed between the larval and adult stages. Of these, several genes were further characterized with quantitative real-time PCR and in situ hybridization, revealing some key findings: 1) vitellogenin was uniquely expressed in late nauplius stage, suggesting it may be an energy source for the subsequent non-feeding cyprid stage; 2) the locations of mannose receptors suggested they may be involved in the sensory system of cyprids; 3) 20 kDa-cement protein homologues were expressed in the cyprid cement gland and probably function during attachment; and 4) receptor tyrosine kinases were expressed higher in cyprid stage and may be involved in signal perception during larval settlement. Conclusions: Our results provide not only the basis of several new hypotheses about gene functions during larval settlement, but also the availability of this large transcriptome dataset in B. amphitrite for further exploration of larval settlement and developmental pathways in this important marine species. © 2011 Chen et al.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rightsChen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS ONEen
dc.subjectmannose receptoren
dc.subjecttyrosine kinase receptoren
dc.subjectvitellogeninen
dc.subjectmessenger RNAen
dc.subjecttranscriptomeen
dc.subjectbarnacleen
dc.subjectcomparative studyen
dc.subjectcontrolled studyen
dc.subjectcypris (larva)en
dc.subjectenergy resourceen
dc.subjectgene expressionen
dc.subjectgene expression profilingen
dc.subjectgene functionen
dc.subjectgenetic identificationen
dc.subjectgenetic transcriptionen
dc.subjectin situ hybridizationen
dc.subjectlarvaen
dc.subjectlarval stageen
dc.subjectmolecular mechanicsen
dc.subjectopen reading frameen
dc.subjectprotein expressionen
dc.subjectpyrosequencingen
dc.subjectreal time polymerase chain reactionen
dc.subjectsequence homologyen
dc.subjectsignal detectionen
dc.subjecttranscriptomicsen
dc.subjectDNA barcodingen
dc.subjectgeneticsen
dc.subjectgrowth, development and agingen
dc.subjectreverse transcription polymerase chain reactionen
dc.subjectThoracicaen
dc.subjectBalanus amphitriteen
dc.subjectCyprididaeen
dc.subjectThoracicaen
dc.subjectDNA Barcoding, Taxonomicen
dc.subjectLarvaen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectRNA, Messengeren
dc.subjectThoracicaen
dc.subjectTranscriptomeen
dc.titleToward an understanding of the molecular mechanisms of barnacle larval settlement: A comparative transcriptomic approachen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3146488en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionWoods Hole Oceanographic Institution, Woods Hole, MA, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAlam, Intikhaben
kaust.authorArcher, John A.C.en
kaust.authorBajic, Vladimir B.en
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