An RNA polymerase II-and AGO4-associated protein acts in RNA-directed DNA methylation

Handle URI:
http://hdl.handle.net/10754/325291
Title:
An RNA polymerase II-and AGO4-associated protein acts in RNA-directed DNA methylation
Authors:
Gao, Zhihuan; Liu, Hai-Liang; Daxinger, Lucia; Pontes, Olga; He, Xinjian; Qian, Weiqiang; Lin, Huixin; Xie, Mingtang; Lorkovic, Zdravko J.; Zhang, ShouDong; Miki, Daisuke; Zhan, Xianqiang; Pontier, Dominique; Lagrange, Thierry; Jin, Hailing; Matzke, Antonius J.; Matzke, Marjori; Pikaard, Craig S.; Zhu, Jian-Kang ( 0000-0001-5134-731X )
Abstract:
DNA methylation is an important epigenetic mark in many eukaryotes. In plants, 24-nucleotide small interfering RNAs (siRNAs) bound to the effector protein, Argonaute 4 (AGO4), can direct de novo DNA methylation by the methyltransferase DRM2 (refs 2, 4-6). Here we report a new regulator of RNA-directed DNA methylation (RdDM) in Arabidopsis: RDM1. Loss-of-function mutations in the RDM1 gene impair the accumulation of 24-nucleotide siRNAs, reduce DNA methylation, and release transcriptional gene silencing at RdDM target loci. RDM1 encodes a small protein that seems to bind single-stranded methyl DNA, and associates and co-localizes with RNA polymerase II (Pol II, also known as NRPB), AGO4 and DRM2 in the nucleus. Our results indicate that RDM1 is a component of the RdDM effector complex and may have a role in linking siRNA production with pre-existing or de novo cytosine methylation. Our results also indicate that, although RDM1 and Pol V (also known as NRPE) may function together at some RdDM target sites in the peri-nucleolar siRNA processing centre, Pol II rather than Pol V is associated with the RdDM effector complex at target sites in the nucleoplasm. © 2010 Macmillan Publishers Limited. All rights reserved.
KAUST Department:
Plant Stress Genomics Research Lab
Citation:
Gao Z, Liu H-L, Daxinger L, Pontes O, He X, et al. (2010) An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 465: 106-109. doi:10.1038/nature09025.
Publisher:
Springer Nature
Journal:
Nature
Issue Date:
21-Apr-2010
DOI:
10.1038/nature09025
PubMed ID:
20410883
PubMed Central ID:
PMC2865564
Type:
Article
ISSN:
00280836
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGao, Zhihuanen
dc.contributor.authorLiu, Hai-Liangen
dc.contributor.authorDaxinger, Luciaen
dc.contributor.authorPontes, Olgaen
dc.contributor.authorHe, Xinjianen
dc.contributor.authorQian, Weiqiangen
dc.contributor.authorLin, Huixinen
dc.contributor.authorXie, Mingtangen
dc.contributor.authorLorkovic, Zdravko J.en
dc.contributor.authorZhang, ShouDongen
dc.contributor.authorMiki, Daisukeen
dc.contributor.authorZhan, Xianqiangen
dc.contributor.authorPontier, Dominiqueen
dc.contributor.authorLagrange, Thierryen
dc.contributor.authorJin, Hailingen
dc.contributor.authorMatzke, Antonius J.en
dc.contributor.authorMatzke, Marjorien
dc.contributor.authorPikaard, Craig S.en
dc.contributor.authorZhu, Jian-Kangen
dc.date.accessioned2014-08-27T09:45:15Zen
dc.date.available2014-08-27T09:45:15Zen
dc.date.issued2010-04-21en
dc.identifier.citationGao Z, Liu H-L, Daxinger L, Pontes O, He X, et al. (2010) An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 465: 106-109. doi:10.1038/nature09025.en
dc.identifier.issn00280836en
dc.identifier.pmid20410883en
dc.identifier.doi10.1038/nature09025en
dc.identifier.urihttp://hdl.handle.net/10754/325291en
dc.description.abstractDNA methylation is an important epigenetic mark in many eukaryotes. In plants, 24-nucleotide small interfering RNAs (siRNAs) bound to the effector protein, Argonaute 4 (AGO4), can direct de novo DNA methylation by the methyltransferase DRM2 (refs 2, 4-6). Here we report a new regulator of RNA-directed DNA methylation (RdDM) in Arabidopsis: RDM1. Loss-of-function mutations in the RDM1 gene impair the accumulation of 24-nucleotide siRNAs, reduce DNA methylation, and release transcriptional gene silencing at RdDM target loci. RDM1 encodes a small protein that seems to bind single-stranded methyl DNA, and associates and co-localizes with RNA polymerase II (Pol II, also known as NRPB), AGO4 and DRM2 in the nucleus. Our results indicate that RDM1 is a component of the RdDM effector complex and may have a role in linking siRNA production with pre-existing or de novo cytosine methylation. Our results also indicate that, although RDM1 and Pol V (also known as NRPE) may function together at some RdDM target sites in the peri-nucleolar siRNA processing centre, Pol II rather than Pol V is associated with the RdDM effector complex at target sites in the nucleoplasm. © 2010 Macmillan Publishers Limited. All rights reserved.en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsUsers may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#termsen
dc.rightsArchived with thanks to Natureen
dc.subjectargonaute proteinen
dc.subjectcytosineen
dc.subjectmethyltransferaseen
dc.subjectnucleotideen
dc.subjectRNA polymerase IIen
dc.subjectsingle stranded DNAen
dc.subjectsmall interfering RNAen
dc.subjectenzymeen
dc.subjecteukaryoteen
dc.subjectgenetic markeren
dc.subjectherben
dc.subjectmethylationen
dc.subjectmutationen
dc.subjectnucleic aciden
dc.subjectpolymerase chain reactionen
dc.subjectproteinen
dc.subjectArabidopsisen
dc.subjectcontrolled studyen
dc.subjectDNA methylationen
dc.subjectDNA RNA hybridizationen
dc.subjectgene locusen
dc.subjectgene silencingen
dc.subjectloss of function mutationen
dc.subjectprotein DNA bindingen
dc.subjectprotein functionen
dc.subjectRNA processingen
dc.subjectArabidopsisen
dc.subjectArabidopsis Proteinsen
dc.subjectCell Nucleusen
dc.subjectDNA Methylationen
dc.subjectDNA-Binding Proteinsen
dc.subjectGene Expression Regulation, Planten
dc.subjectGene Silencingen
dc.subjectMethyltransferasesen
dc.subjectMutationen
dc.subjectRNA Polymerase IIen
dc.subjectRNA, Planten
dc.subjectArabidopsisen
dc.subjectEukaryotaen
dc.titleAn RNA polymerase II-and AGO4-associated protein acts in RNA-directed DNA methylationen
dc.typeArticleen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalNatureen
dc.identifier.pmcidPMC2865564en
dc.eprint.versionPost-printen
dc.contributor.institutionInstitute for Integrative Genome Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, United Statesen
dc.contributor.institutionSchool of Life Science and Technology, Tongji University, Shanghai 200092, Chinaen
dc.contributor.institutionGregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, 1030 Vienna, Austriaen
dc.contributor.institutionBiology Department, Washington University, St Louis, MI 63130, United Statesen
dc.contributor.institutionMax F. Perutz Laboratory, Medical University of Vienna, 1030 Vienna, Austriaen
dc.contributor.institutionLGDP, CNRS/IRD/Université de Perpignan, UMR 5096, 66860 Perpignan, Franceen
dc.contributor.institutionInstitute for Integrative Genome Biology, Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521, United Statesen
dc.contributor.institutionDepartment of Biology, Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, United Statesen
dc.contributor.institutionDivision of Genetics and Population Health, Queensland Institute of Medical Research, QLD 4006, Herston, Australiaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorHe, Xinjianen
kaust.authorZhang, ShouDongen
kaust.authorZhan, Xiangqiangen
kaust.authorZhu, Jian-Kangen

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