A eukaryotic-acquired gene by a biotrophic phytopathogen allows prolonged survival on the host by counteracting the shut-down of plant photosynthesis

Handle URI:
http://hdl.handle.net/10754/325283
Title:
A eukaryotic-acquired gene by a biotrophic phytopathogen allows prolonged survival on the host by counteracting the shut-down of plant photosynthesis
Authors:
Garavaglia, Betiana S.; Thomas, Ludivine; Gottig, Natalia; Dunger, Germán; Garofalo, Cecilia G.; Daurelio, Lucas D.; Ndimba, Bongani; Orellano, Elena G.; Gehring, Christoph A. ( 0000-0003-4355-4591 ) ; Ottado, Jorgelina
Abstract:
Xanthomonas citri pv. citri, the bacteria responsible for citrus canker posses a biological active plant natriuretic peptide (PNP)-like protein, not present in any other bacteria. PNPs are a class of extracellular, systemically mobile peptides that elicit a number of plant responses important in homeostasis and growth. Previously, we showed that a Xanthomonas citri pv. citri mutant lacking the PNP-like protein XacPNP produced more necrotic lesions in citrus leaves than wild type infections and suggested a role for XacPNP in the regulation of host homeostasis. Here we have analyzed the proteome modifications observed in citrus leaves infected with the wild type and XacPNP deletion mutant bacteria. While both of them cause downregulation of enzymes related to photosynthesis as well as chloroplastic ribosomal proteins, proteins related to defense responses are up-regulated. However, leaves infiltrated with the XacPNP deletion mutant show a more pronounced decrease in photosynthetic proteins while no reduction in defense related proteins as compared to the wild-type pathogen. This suggests that XacPNP serves the pathogen to maintain host photosynthetic efficiency during pathogenesis. The results from the proteomics analyses are consistent with our chlorophyll fluorescence data and transcript analyses of defense genes that show a more marked reduction in photosynthesis in the mutant but no difference in the induction of genes diagnostic for biotic-stress responses. We therefore conclude that XacPNP counteracts the shut-down of host photosynthesis during infection and in that way maintains the tissue in better conditions, suggesting that the pathogen has adapted a host gene to modify its natural host and render it a better reservoir for prolonged bacterial survival and thus for further colonization. 2010 Garavaglia et al.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Citation:
Garavaglia BS, Thomas L, Gottig N, Dunger G, Garofalo CG, et al. (2010) A Eukaryotic-Acquired Gene by a Biotrophic Phytopathogen Allows Prolonged Survival on the Host by Counteracting the Shut-Down of Plant Photosynthesis. PLoS ONE 5: e8950. doi:10.1371/journal.pone.0008950.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
28-Jan-2010
DOI:
10.1371/journal.pone.0008950
PubMed ID:
20126632
PubMed Central ID:
PMC2812515
Type:
Article
ISSN:
19326203
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGaravaglia, Betiana S.en
dc.contributor.authorThomas, Ludivineen
dc.contributor.authorGottig, Nataliaen
dc.contributor.authorDunger, Germánen
dc.contributor.authorGarofalo, Cecilia G.en
dc.contributor.authorDaurelio, Lucas D.en
dc.contributor.authorNdimba, Bonganien
dc.contributor.authorOrellano, Elena G.en
dc.contributor.authorGehring, Christoph A.en
dc.contributor.authorOttado, Jorgelinaen
dc.date.accessioned2014-08-27T09:44:49Z-
dc.date.available2014-08-27T09:44:49Z-
dc.date.issued2010-01-28en
dc.identifier.citationGaravaglia BS, Thomas L, Gottig N, Dunger G, Garofalo CG, et al. (2010) A Eukaryotic-Acquired Gene by a Biotrophic Phytopathogen Allows Prolonged Survival on the Host by Counteracting the Shut-Down of Plant Photosynthesis. PLoS ONE 5: e8950. doi:10.1371/journal.pone.0008950.en
dc.identifier.issn19326203en
dc.identifier.pmid20126632en
dc.identifier.doi10.1371/journal.pone.0008950en
dc.identifier.urihttp://hdl.handle.net/10754/325283en
dc.description.abstractXanthomonas citri pv. citri, the bacteria responsible for citrus canker posses a biological active plant natriuretic peptide (PNP)-like protein, not present in any other bacteria. PNPs are a class of extracellular, systemically mobile peptides that elicit a number of plant responses important in homeostasis and growth. Previously, we showed that a Xanthomonas citri pv. citri mutant lacking the PNP-like protein XacPNP produced more necrotic lesions in citrus leaves than wild type infections and suggested a role for XacPNP in the regulation of host homeostasis. Here we have analyzed the proteome modifications observed in citrus leaves infected with the wild type and XacPNP deletion mutant bacteria. While both of them cause downregulation of enzymes related to photosynthesis as well as chloroplastic ribosomal proteins, proteins related to defense responses are up-regulated. However, leaves infiltrated with the XacPNP deletion mutant show a more pronounced decrease in photosynthetic proteins while no reduction in defense related proteins as compared to the wild-type pathogen. This suggests that XacPNP serves the pathogen to maintain host photosynthetic efficiency during pathogenesis. The results from the proteomics analyses are consistent with our chlorophyll fluorescence data and transcript analyses of defense genes that show a more marked reduction in photosynthesis in the mutant but no difference in the induction of genes diagnostic for biotic-stress responses. We therefore conclude that XacPNP counteracts the shut-down of host photosynthesis during infection and in that way maintains the tissue in better conditions, suggesting that the pathogen has adapted a host gene to modify its natural host and render it a better reservoir for prolonged bacterial survival and thus for further colonization. 2010 Garavaglia et al.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsGaravaglia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS ONEen
dc.subjectnatriuretic factoren
dc.subjectproteomeen
dc.subjectribosome proteinen
dc.subjectvegetable proteinen
dc.subjectabiotic stressen
dc.subjectbacterial infectionen
dc.subjectbacterial survivalen
dc.subjectbiotic stressen
dc.subjectcarbon metabolismen
dc.subjectchlorophyll fluorescenceen
dc.subjectcontrolled studyen
dc.subjectdeletion mutanten
dc.subjectdown regulationen
dc.subjecteukaryoteen
dc.subjecthomeostasisen
dc.subjectinnate immunityen
dc.subjectnatural hosten
dc.subjectnucleotide sequenceen
dc.subjectpathogenesisen
dc.subjectphotosynthesisen
dc.subjectplant leafen
dc.subjectplant responseen
dc.subjectprotein modificationen
dc.subjectsweet orangeen
dc.subjectultraviolet radiationen
dc.subjectupregulationen
dc.subjectXanthomonasen
dc.subjectxanthomonas citrien
dc.subjectCitrusen
dc.subjectElectrophoresis, Gel, Two-Dimensionalen
dc.subjectGenes, Planten
dc.subjectHomeostasisen
dc.subjectPhotosynthesisen
dc.subjectPlant Leavesen
dc.subjectPlant Proteinsen
dc.subjectProteomeen
dc.subjectSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen
dc.subjectXanthomonasen
dc.subjectBacteria (microorganisms)en
dc.subjectCitrusen
dc.subjectEukaryotaen
dc.subjectXanthomonas citrien
dc.titleA eukaryotic-acquired gene by a biotrophic phytopathogen allows prolonged survival on the host by counteracting the shut-down of plant photosynthesisen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC2812515en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionMolecular Biology Division, Instituto de Biologa Molecular Y Celular de Rosario, Universidad Nacional de Rosario, Rosario, Argentinaen
dc.contributor.institutionConsejo de Investigaciones, Universidad Nacional de Rosario, Rosario, Argentinaen
dc.contributor.institutionDepartment of Biotechnology, University of the Western Cape, Bellville, South Africaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorGehring, Christoph A.en

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