Dependency on de novo protein synthesis and proteomic changes during metamorphosis of the marine bryozoan Bugula neritina

Handle URI:
http://hdl.handle.net/10754/325256
Title:
Dependency on de novo protein synthesis and proteomic changes during metamorphosis of the marine bryozoan Bugula neritina
Authors:
Wong, Yue Him; Arellano, Shawn M; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Qian, Pei-Yuan
Abstract:
Background: Metamorphosis in the bryozoan Bugula neritina (Linne) includes an initial phase of rapid morphological rearrangement followed by a gradual phase of morphogenesis. We hypothesized that the first phase may be independent of de novo synthesis of proteins and, instead, involves post-translational modifications of existing proteins, providing a simple mechanism to quickly initiate metamorphosis. To test our hypothesis, we challenged B. neritina larvae with transcription and translation inhibitors. Furthermore, we employed 2D gel electrophoresis to characterize changes in the phosphoproteome and proteome during early metamorphosis. Differentially expressed proteins were identified by liquid chromatography tandem mass spectrometry and their gene expression patterns were profiled using semi-quantitative real time PCR.Results: When larvae were incubated with transcription and translation inhibitors, metamorphosis initiated through the first phase but did not complete. We found a significant down-regulation of 60 protein spots and the percentage of phosphoprotein spots decreased from 15% in the larval stage to12% during early metamorphosis. Two proteins--the mitochondrial processing peptidase beta subunit and severin--were abundantly expressed and phosphorylated in the larval stage, but down-regulated during metamorphosis. MPPbeta and severin were also down-regulated on the gene expression level.Conclusions: The initial morphogenetic changes that led to attachment of B. neritina did not depend on de novo protein synthesis, but the subsequent gradual morphogenesis did. This is the first time that the mitochondrial processing peptidase beta subunit or severin have been shown to be down-regulated on both gene and protein expression levels during the metamorphosis of B. neritina. Future studies employing immunohistochemistry to reveal the expression locality of these two proteins during metamorphosis should provide further evidence of the involvement of these two proteins in the morphogenetic rearrangement of B. neritina. 2010 Wong et al; licensee BioMed Central Ltd.
KAUST Department:
Biosciences Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC)
Citation:
Wong Y, Arellano SM, Zhang H, Ravasi T, Qian P-Y (2010) Dependency on de novo protein synthesis and proteomic changes during metamorphosis of the marine bryozoan Bugula neritina. Proteome Science 8: 25. doi:10.1186/1477-5956-8-25.
Publisher:
BioMed Central
Journal:
Proteome Science
Issue Date:
24-May-2010
DOI:
10.1186/1477-5956-8-25
PubMed ID:
20497544
PubMed Central ID:
PMC2890537
Type:
Article
ISSN:
14775956
Appears in Collections:
Articles; Bioscience Program; Biosciences Core Lab; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWong, Yue Himen
dc.contributor.authorArellano, Shawn Men
dc.contributor.authorZhang, Huomingen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-08-27T09:42:51Z-
dc.date.available2014-08-27T09:42:51Z-
dc.date.issued2010-05-24en
dc.identifier.citationWong Y, Arellano SM, Zhang H, Ravasi T, Qian P-Y (2010) Dependency on de novo protein synthesis and proteomic changes during metamorphosis of the marine bryozoan Bugula neritina. Proteome Science 8: 25. doi:10.1186/1477-5956-8-25.en
dc.identifier.issn14775956en
dc.identifier.pmid20497544en
dc.identifier.doi10.1186/1477-5956-8-25en
dc.identifier.urihttp://hdl.handle.net/10754/325256en
dc.description.abstractBackground: Metamorphosis in the bryozoan Bugula neritina (Linne) includes an initial phase of rapid morphological rearrangement followed by a gradual phase of morphogenesis. We hypothesized that the first phase may be independent of de novo synthesis of proteins and, instead, involves post-translational modifications of existing proteins, providing a simple mechanism to quickly initiate metamorphosis. To test our hypothesis, we challenged B. neritina larvae with transcription and translation inhibitors. Furthermore, we employed 2D gel electrophoresis to characterize changes in the phosphoproteome and proteome during early metamorphosis. Differentially expressed proteins were identified by liquid chromatography tandem mass spectrometry and their gene expression patterns were profiled using semi-quantitative real time PCR.Results: When larvae were incubated with transcription and translation inhibitors, metamorphosis initiated through the first phase but did not complete. We found a significant down-regulation of 60 protein spots and the percentage of phosphoprotein spots decreased from 15% in the larval stage to12% during early metamorphosis. Two proteins--the mitochondrial processing peptidase beta subunit and severin--were abundantly expressed and phosphorylated in the larval stage, but down-regulated during metamorphosis. MPPbeta and severin were also down-regulated on the gene expression level.Conclusions: The initial morphogenetic changes that led to attachment of B. neritina did not depend on de novo protein synthesis, but the subsequent gradual morphogenesis did. This is the first time that the mitochondrial processing peptidase beta subunit or severin have been shown to be down-regulated on both gene and protein expression levels during the metamorphosis of B. neritina. Future studies employing immunohistochemistry to reveal the expression locality of these two proteins during metamorphosis should provide further evidence of the involvement of these two proteins in the morphogenetic rearrangement of B. neritina. 2010 Wong et al; licensee BioMed Central Ltd.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en
dc.subjectF actinen
dc.subjectmitochondrial processing peptidase beta subuniten
dc.subjectmitochondrial proteinen
dc.subjectseverinen
dc.subjectunclassified drugen
dc.subjectBryozoaen
dc.subjectBugula neritinaen
dc.subjectcontrolled studyen
dc.subjectdown regulationen
dc.subjectgene expression profilingen
dc.subjectlarval stageen
dc.subjectliquid chromatographyen
dc.subjectmarine speciesen
dc.subjectmetamorphosisen
dc.subjectprotein localizationen
dc.subjectprotein phosphorylationen
dc.subjectprotein processingen
dc.subjectprotein synthesisen
dc.subjectproteomicsen
dc.subjectreal time polymerase chain reactionen
dc.subjecttandem mass spectrometryen
dc.subjecttwo dimensional gel electrophoresisen
dc.subjectBryozoaen
dc.subjectBugula neritinaen
dc.subjectGymnolaemataen
dc.titleDependency on de novo protein synthesis and proteomic changes during metamorphosis of the marine bryozoan Bugula neritinaen
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalProteome Scienceen
dc.identifier.pmcidPMC2890537en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorZhang, Huomingen
kaust.authorRavasi, Timothyen
This item is licensed under a Creative Commons License
Creative Commons
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.