Unique prokaryotic consortia in geochemically distinct sediments from Red Sea Atlantis II and discovery deep brine pools.

Handle URI:
http://hdl.handle.net/10754/323524
Title:
Unique prokaryotic consortia in geochemically distinct sediments from Red Sea Atlantis II and discovery deep brine pools.
Authors:
Siam, Rania; Mustafa, Ghada A; Sharaf, Hazem; Moustafa, Ahmed; Ramadan, Adham R; Antunes, Andre; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Stingl, Ulrich ( 0000-0002-0684-2597 ) ; Marsis, Nardine G R; Coolen, Marco J L; Sogin, Mitchell; Ferreira, Ari J S; Dorry, Hamza El
Abstract:
The seafloor is a unique environment, which allows insights into how geochemical processes affect the diversity of biological life. Among its diverse ecosystems are deep-sea brine pools - water bodies characterized by a unique combination of extreme conditions. The 'polyextremophiles' that constitute the microbial assemblage of these deep hot brines have not been comprehensively studied. We report a comparative taxonomic analysis of the prokaryotic communities of the sediments directly below the Red Sea brine pools, namely, Atlantis II, Discovery, Chain Deep, and an adjacent brine-influenced site. Analyses of sediment samples and high-throughput pyrosequencing of PCR-amplified environmental 16S ribosomal RNA genes (16S rDNA) revealed that one sulfur (S)-rich Atlantis II and one nitrogen (N)-rich Discovery Deep section contained distinct microbial populations that differed from those found in the other sediment samples examined. Proteobacteria, Actinobacteria, Cyanobacteria, Deferribacteres, and Euryarchaeota were the most abundant bacterial and archaeal phyla in both the S- and N-rich sections. Relative abundance-based hierarchical clustering of the 16S rDNA pyrotags assigned to major taxonomic groups allowed us to categorize the archaeal and bacterial communities into three major and distinct groups; group I was unique to the S-rich Atlantis II section (ATII-1), group II was characteristic for the N-rich Discovery sample (DD-1), and group III reflected the composition of the remaining sediments. Many of the groups detected in the S-rich Atlantis II section are likely to play a dominant role in the cycling of methane and sulfur due to their phylogenetic affiliations with bacteria and archaea involved in anaerobic methane oxidation and sulfate reduction.
KAUST Department:
Red Sea Research Center (RSRC); Computational Bioscience Research Center (CBRC)
Citation:
Siam R, Mustafa GA, Sharaf H, Moustafa A, Ramadan AR, et al. (2012) Unique Prokaryotic Consortia in Geochemically Distinct Sediments from Red Sea Atlantis II and Discovery Deep Brine Pools. PLoS ONE 7: e42872. doi:10.1371/journal.pone.0042872.
Publisher:
Public Library of Science
Journal:
PloS one
Issue Date:
20-Aug-2012
DOI:
10.1371/journal.pone.0042872
PubMed ID:
22916172
PubMed Central ID:
PMC3423430
Type:
Article
ISSN:
1932-6203
Additional Links:
http://europepmc.org/abstract/MED/22916172; http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042872
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorSiam, Raniaen
dc.contributor.authorMustafa, Ghada Aen
dc.contributor.authorSharaf, Hazemen
dc.contributor.authorMoustafa, Ahmeden
dc.contributor.authorRamadan, Adham Ren
dc.contributor.authorAntunes, Andreen
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorStingl, Ulrichen
dc.contributor.authorMarsis, Nardine G Ren
dc.contributor.authorCoolen, Marco J Len
dc.contributor.authorSogin, Mitchellen
dc.contributor.authorFerreira, Ari J Sen
dc.contributor.authorDorry, Hamza Elen
dc.date.accessioned2014-07-21T10:00:41Z-
dc.date.available2014-07-21T10:00:41Z-
dc.date.issued2012-08-20en
dc.identifier.citationSiam R, Mustafa GA, Sharaf H, Moustafa A, Ramadan AR, et al. (2012) Unique Prokaryotic Consortia in Geochemically Distinct Sediments from Red Sea Atlantis II and Discovery Deep Brine Pools. PLoS ONE 7: e42872. doi:10.1371/journal.pone.0042872.en
dc.identifier.issn1932-6203en
dc.identifier.pmid22916172en
dc.identifier.doi10.1371/journal.pone.0042872en
dc.identifier.urihttp://hdl.handle.net/10754/323524en
dc.description.abstractThe seafloor is a unique environment, which allows insights into how geochemical processes affect the diversity of biological life. Among its diverse ecosystems are deep-sea brine pools - water bodies characterized by a unique combination of extreme conditions. The 'polyextremophiles' that constitute the microbial assemblage of these deep hot brines have not been comprehensively studied. We report a comparative taxonomic analysis of the prokaryotic communities of the sediments directly below the Red Sea brine pools, namely, Atlantis II, Discovery, Chain Deep, and an adjacent brine-influenced site. Analyses of sediment samples and high-throughput pyrosequencing of PCR-amplified environmental 16S ribosomal RNA genes (16S rDNA) revealed that one sulfur (S)-rich Atlantis II and one nitrogen (N)-rich Discovery Deep section contained distinct microbial populations that differed from those found in the other sediment samples examined. Proteobacteria, Actinobacteria, Cyanobacteria, Deferribacteres, and Euryarchaeota were the most abundant bacterial and archaeal phyla in both the S- and N-rich sections. Relative abundance-based hierarchical clustering of the 16S rDNA pyrotags assigned to major taxonomic groups allowed us to categorize the archaeal and bacterial communities into three major and distinct groups; group I was unique to the S-rich Atlantis II section (ATII-1), group II was characteristic for the N-rich Discovery sample (DD-1), and group III reflected the composition of the remaining sediments. Many of the groups detected in the S-rich Atlantis II section are likely to play a dominant role in the cycling of methane and sulfur due to their phylogenetic affiliations with bacteria and archaea involved in anaerobic methane oxidation and sulfate reduction.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urlhttp://europepmc.org/abstract/MED/22916172en
dc.relation.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042872en
dc.rightsArchived with thanks to PloS oneen
dc.subject.meshArchaeaen
dc.subject.meshBacteriaen
dc.subject.meshGeologic Sedimentsen
dc.subject.meshSeawateren
dc.subject.meshSpecies Specificityen
dc.subject.meshArchaeaen
dc.subject.meshBacteriaen
dc.subject.meshGeologic Sedimentsen
dc.subject.meshSeawateren
dc.subject.meshSpecies Specificityen
dc.titleUnique prokaryotic consortia in geochemically distinct sediments from Red Sea Atlantis II and discovery deep brine pools.en
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalPloS oneen
dc.identifier.pmcidPMC3423430en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionBiotechnology Graduate Program, American University in Cairo, Cairo, Egypt. rsiam@aucegypt.eduen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAntunes, Andreen
kaust.authorBajic, Vladimir B.en
kaust.authorStingl, Ulrichen

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