Transcriptomic responses to heat stress and bleaching in the elkhorn coral Acropora palmata

Handle URI:
http://hdl.handle.net/10754/323512
Title:
Transcriptomic responses to heat stress and bleaching in the elkhorn coral Acropora palmata
Authors:
DeSalvo, MK; Sunagawa, S; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Medina, M
Abstract:
The emergence of genomic tools for reef-building corals and symbiotic anemones comes at a time when alarming losses in coral cover are being observed worldwide. These tools hold great promise in elucidating novel and unforeseen cellular processes underlying the successful mutualism between corals and their dinoflagellate endosymbionts Symbiodinium spp. Since thermal stress triggers a breakdown in the symbiosis (coral bleaching), measuring the transcriptomic response to thermal stress-induced bleaching offers an extraordinary view of cellular processes that are specific to coral–algal symbioses. In the present study, we utilized a cDNA microarray containing 2059 genes of the threatened Caribbean elkhorn coral Acropora palmata to identify genes that are differentially expressed upon thermal stress. Fragments from replicate colonies were exposed to elevated temperature for 2 d, and samples were frozen for microarray analysis after 24 and 48 h. Totals of 204 and 104 genes were differentially expressed in samples that were collected 1 and 2 d after thermal stress, respectively. Analysis of the differentially expressed genes indicates a cellular stress response in A. palmata involving (1) growth arrest, (2) chaperone activity, (3) nucleic acid stabilization and repair, and (4) removal of damaged macromolecules. Other differentially expressed processes include sensory perception, metabolite transfer between host and endosymbiont, nitric oxide signaling, and modifications to the actin cytoskeleton and extracellular matrix. The results are compared with those from a previous coral microarray study of thermal stress in Montastraea faveolata, and point to an overall evolutionary conserved bleaching response in scleractinian corals.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
DeSalvo M, Sunagawa S, Voolstra C, Medina M (2010) Transcriptomic responses to heat stress and bleaching in the elkhorn coral Acropora palmata. Mar Ecol Prog Ser 402: 97-113. doi:10.3354/meps08372.
Publisher:
Inter-Research Science Center
Journal:
Marine Ecology Progress Series
Issue Date:
8-Mar-2010
DOI:
10.3354/meps08372
Type:
Article
ISSN:
0171-8630; 1616-1599
Additional Links:
http://www.int-res.com/abstracts/meps/v402/p97-113/
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorDeSalvo, MKen
dc.contributor.authorSunagawa, Sen
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorMedina, Men
dc.date.accessioned2014-07-21T11:48:54Z-
dc.date.available2014-07-21T11:48:54Z-
dc.date.issued2010-03-08en
dc.identifier.citationDeSalvo M, Sunagawa S, Voolstra C, Medina M (2010) Transcriptomic responses to heat stress and bleaching in the elkhorn coral Acropora palmata. Mar Ecol Prog Ser 402: 97-113. doi:10.3354/meps08372.en
dc.identifier.issn0171-8630en
dc.identifier.issn1616-1599en
dc.identifier.doi10.3354/meps08372en
dc.identifier.urihttp://hdl.handle.net/10754/323512en
dc.description.abstractThe emergence of genomic tools for reef-building corals and symbiotic anemones comes at a time when alarming losses in coral cover are being observed worldwide. These tools hold great promise in elucidating novel and unforeseen cellular processes underlying the successful mutualism between corals and their dinoflagellate endosymbionts Symbiodinium spp. Since thermal stress triggers a breakdown in the symbiosis (coral bleaching), measuring the transcriptomic response to thermal stress-induced bleaching offers an extraordinary view of cellular processes that are specific to coral–algal symbioses. In the present study, we utilized a cDNA microarray containing 2059 genes of the threatened Caribbean elkhorn coral Acropora palmata to identify genes that are differentially expressed upon thermal stress. Fragments from replicate colonies were exposed to elevated temperature for 2 d, and samples were frozen for microarray analysis after 24 and 48 h. Totals of 204 and 104 genes were differentially expressed in samples that were collected 1 and 2 d after thermal stress, respectively. Analysis of the differentially expressed genes indicates a cellular stress response in A. palmata involving (1) growth arrest, (2) chaperone activity, (3) nucleic acid stabilization and repair, and (4) removal of damaged macromolecules. Other differentially expressed processes include sensory perception, metabolite transfer between host and endosymbiont, nitric oxide signaling, and modifications to the actin cytoskeleton and extracellular matrix. The results are compared with those from a previous coral microarray study of thermal stress in Montastraea faveolata, and point to an overall evolutionary conserved bleaching response in scleractinian corals.en
dc.language.isoenen
dc.publisherInter-Research Science Centeren
dc.relation.urlhttp://www.int-res.com/abstracts/meps/v402/p97-113/en
dc.rightsArchived with thanks to Marine Ecology Progress Seriesen
dc.titleTranscriptomic responses to heat stress and bleaching in the elkhorn coral Acropora palmataen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalMarine Ecology Progress Seriesen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionSchool of Natural Sciences, University of California Merced, Merced, CA 95343, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorVoolstra, Christian R.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.