Handle URI:
http://hdl.handle.net/10754/304731
Title:
Microbead agglutination based assays
Authors:
Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Castro, David; Foulds, Ian G.; Parameswaran, Ash M.; Sumanpreet, K. Chhina
Abstract:
We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Publisher:
KAUST Winter Enrichment Program (WEP) 2013
Journal:
KAUST Winter Enrichment Program (WEP) 2013
Issue Date:
21-Jan-2013
Type:
Poster
Additional Links:
http://www.acadox.com/class/1064#info
Appears in Collections:
Posters; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorCastro, Daviden
dc.contributor.authorFoulds, Ian G.en
dc.contributor.authorParameswaran, Ash M.en
dc.contributor.authorSumanpreet, K. Chhinaen
dc.date.accessioned2013-10-30T06:05:22Z-
dc.date.available2013-10-30T06:05:22Z-
dc.date.issued2013-01-21en
dc.identifier.urihttp://hdl.handle.net/10754/304731en
dc.description.abstractWe report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.en
dc.language.isoenen
dc.publisherKAUST Winter Enrichment Program (WEP) 2013en
dc.relation.urlhttp://www.acadox.com/class/1064#infoen
dc.subjectAgglutinationen
dc.subjectMicrobeadsen
dc.subjectDNA detectionen
dc.subjectMicrofluidicsen
dc.subjectDNA hybridizationen
dc.titleMicrobead agglutination based assaysen
dc.typePosteren
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalKAUST Winter Enrichment Program (WEP) 2013en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
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