Apparatus, System and Method for Fast Detection of Genetic Information by PCR in an Interchangeable Chip

Handle URI:
http://hdl.handle.net/10754/304710
Title:
Apparatus, System and Method for Fast Detection of Genetic Information by PCR in an Interchangeable Chip
Authors:
Wen, Weijia; Wu, Jinbo; Kodzius, Rimantas ( 0000-0001-9417-8894 )
Abstract:
A polymerase chain reaction (PCR) device for fast amplification and detection of DNA includes an interchangeable PCR chamber, a temperature control component, and an optical detection system. The DNA amplification is performed on an interchangeable chip with volumes as small as 1.25 µl, while the heating and cooling rate may be as fast as 12.7 °C/second ensuring that the total time needed of only 25 minutes to complete the 35 cycle PCR amplification. The PCR may be performed according to a two-temperature approach for denaturing and annealing (Td and Ta) of DNA with the PCR chip, with which the amplification of male-specific SRY gene marker by utilizing raw saliva may be achieved. The genetic identification may be in-situ detected after PCR by the optical detection system.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Publisher:
United States Patent and Trademark Office
Issue Date:
3-Mar-2011
Submitted date:
2011-03-03
Type:
Patent
Patent Status:
Provisional Application
Appears in Collections:
Patents; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorWen, Weijiaen
dc.contributor.authorWu, Jinboen
dc.contributor.authorKodzius, Rimantasen
dc.date.accessioned2013-10-30T07:05:25Zen
dc.date.available2013-10-30T07:05:25Zen
dc.date.issued2011-03-03en
dc.date.submitted2011-03-03en
dc.identifier.urihttp://hdl.handle.net/10754/304710en
dc.description.abstractA polymerase chain reaction (PCR) device for fast amplification and detection of DNA includes an interchangeable PCR chamber, a temperature control component, and an optical detection system. The DNA amplification is performed on an interchangeable chip with volumes as small as 1.25 µl, while the heating and cooling rate may be as fast as 12.7 °C/second ensuring that the total time needed of only 25 minutes to complete the 35 cycle PCR amplification. The PCR may be performed according to a two-temperature approach for denaturing and annealing (Td and Ta) of DNA with the PCR chip, with which the amplification of male-specific SRY gene marker by utilizing raw saliva may be achieved. The genetic identification may be in-situ detected after PCR by the optical detection system.en
dc.language.isoenen
dc.publisherUnited States Patent and Trademark Officeen
dc.subjectPCR (polymerase chain reaction)en
dc.subjectDNA detectionen
dc.subjectMicrofluidicsen
dc.subjectinterchangeable chipen
dc.subjecttwo-temperature PCRen
dc.titleApparatus, System and Method for Fast Detection of Genetic Information by PCR in an Interchangeable Chipen
dc.typePatenten
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.description.statusProvisional Applicationen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionThe Hong Kong University of Science and Technology (HKUST)en
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
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