Material Biocompatibility for PCR Microfluidic Chips

Handle URI:
http://hdl.handle.net/10754/304707
Title:
Material Biocompatibility for PCR Microfluidic Chips
Authors:
Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Chang, Donald Choy; Gong, Xiuqing; Wen, Weijia; Wu, Jinbo; Xiao, Kang; Yi, Xin
Abstract:
As part of the current miniaturization trend, biological reactions and processes are being adapted to microfluidics devices. PCR is the primary method employed in DNA amplification, its miniaturization is central to efforts to develop portable devices for diagnostics and testing purposes. A problem is the PCR-inhibitory effect due to interaction between PCR reagents and the surrounding environment, which effect is increased in high-surface-are-to-volume ration microfluidics. In this study, we evaluated the biocompatibility of various common materials employed in the fabrication of microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most of the cases, addition of bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, whereas they did show noticeable interaction with the DNA polymerase. Our test, instead of using microfluidic devices, can be easily conducted in common PCR tubes using a standard bench thermocycler. Our data supports an overview of the means by which the materials most bio-friendly to microfluidics can be selected.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Publisher:
Hong Kong University of Science and Technology
Journal:
Hong Kong University of Science and Technology
Issue Date:
23-Apr-2010
Type:
Poster
Sponsors:
Hong Kong Research Grants Council (Grant No. HKUST 603208 and 660207); Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST)
Additional Links:
http://pgworkshop.ust.hk/
Appears in Collections:
Posters; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorChang, Donald Choyen
dc.contributor.authorGong, Xiuqingen
dc.contributor.authorWen, Weijiaen
dc.contributor.authorWu, Jinboen
dc.contributor.authorXiao, Kangen
dc.contributor.authorYi, Xinen
dc.date.accessioned2013-10-30T06:09:42Z-
dc.date.available2013-10-30T06:09:42Z-
dc.date.issued2010-04-23en
dc.identifier.urihttp://hdl.handle.net/10754/304707en
dc.description.abstractAs part of the current miniaturization trend, biological reactions and processes are being adapted to microfluidics devices. PCR is the primary method employed in DNA amplification, its miniaturization is central to efforts to develop portable devices for diagnostics and testing purposes. A problem is the PCR-inhibitory effect due to interaction between PCR reagents and the surrounding environment, which effect is increased in high-surface-are-to-volume ration microfluidics. In this study, we evaluated the biocompatibility of various common materials employed in the fabrication of microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most of the cases, addition of bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, whereas they did show noticeable interaction with the DNA polymerase. Our test, instead of using microfluidic devices, can be easily conducted in common PCR tubes using a standard bench thermocycler. Our data supports an overview of the means by which the materials most bio-friendly to microfluidics can be selected.en
dc.description.sponsorshipHong Kong Research Grants Council (Grant No. HKUST 603208 and 660207); Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST)en
dc.language.isoenen
dc.publisherHong Kong University of Science and Technologyen
dc.relation.urlhttp://pgworkshop.ust.hk/en
dc.subjectPolymerase chain reaction (PCR)en
dc.subjectDNA amplificationen
dc.subjectPCR compatibilityen
dc.subjectBiocompatibilityen
dc.titleMaterial Biocompatibility for PCR Microfluidic Chipsen
dc.typePosteren
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalHong Kong University of Science and Technologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionThe Hong Kong University of Science and Technology (HKUST)en
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
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