Towards a high throughput droplet-based agglutination assay

Handle URI:
http://hdl.handle.net/10754/303787
Title:
Towards a high throughput droplet-based agglutination assay
Authors:
Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Castro, David; Foulds, Ian G.
Abstract:
This work demonstrates the detection method for a high throughput droplet based agglutination assay system. Using simple hydrodynamic forces to mix and aggregate functionalized microbeads we avoid the need to use magnetic assistance or mixing structures. The concentration of our target molecules was estimated by agglutination strength, obtained through optical image analysis. Agglutination in droplets was performed with flow rates of 150 µl/min and occurred in under a minute, with potential to perform high-throughput measurements. The lowest target concentration detected in droplet microfluidics was 0.17 nM, which is three orders of magnitude more sensitive than a conventional card based agglutination assay.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Publisher:
The 7th International Conference on Microtechnologies in Medicine and Biology MMB
Journal:
The 7th International Conference on Microtechnologies in Medicine and Biology MMB
Issue Date:
22-Oct-2013
Type:
Conference Paper; Poster
Additional Links:
ftp://pmmimeetings.com/MMB_Manuscripts/0119_MMB_Kodzius_1.pdf
Appears in Collections:
Conference Papers; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorCastro, Daviden
dc.contributor.authorFoulds, Ian G.en
dc.date.accessioned2013-10-22T08:37:04Z-
dc.date.available2013-10-22T08:37:04Z-
dc.date.issued2013-10-22en
dc.identifier.urihttp://hdl.handle.net/10754/303787en
dc.description.abstractThis work demonstrates the detection method for a high throughput droplet based agglutination assay system. Using simple hydrodynamic forces to mix and aggregate functionalized microbeads we avoid the need to use magnetic assistance or mixing structures. The concentration of our target molecules was estimated by agglutination strength, obtained through optical image analysis. Agglutination in droplets was performed with flow rates of 150 µl/min and occurred in under a minute, with potential to perform high-throughput measurements. The lowest target concentration detected in droplet microfluidics was 0.17 nM, which is three orders of magnitude more sensitive than a conventional card based agglutination assay.en
dc.language.isoenen
dc.publisherThe 7th International Conference on Microtechnologies in Medicine and Biology MMBen
dc.relation.urlftp://pmmimeetings.com/MMB_Manuscripts/0119_MMB_Kodzius_1.pdfen
dc.subjectAgglutinationen
dc.subjectMicrobeadsen
dc.subjectMicrodropletsen
dc.subjectBiological recognitionen
dc.subjectPoint-of-Care testingen
dc.titleTowards a high throughput droplet-based agglutination assayen
dc.typeConference Paperen
dc.typePosteren
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalThe 7th International Conference on Microtechnologies in Medicine and Biology MMBen
dc.eprint.versionPublisher's Version/PDFen
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