Inhibitory effect of common microfluidic materials on PCR outcome

Handle URI:
http://hdl.handle.net/10754/303149
Title:
Inhibitory effect of common microfluidic materials on PCR outcome
Authors:
Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Xiao, Kang; Wu, Jinbo; Yi, Xin; Gong, Xiuqing; Foulds, Ian G.; Wen, Weijia
Abstract:
In this study, we established a simple method for evaluating the PCR compatibility of various common materials employed when fabricating microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most cases, adding bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, although they noticeably interacted with the polymerase. We provide a simple method of performing PCR-compatibility testing of materials using inexpensive instrumentation that is common in molecular biology laboratories. Furthermore, our method is direct, being performed under actual PCR conditions with high temperature. Our results provide an overview of materials that are PCR-friendly for fabricating microfluidic devices. The PCR reaction, without any additives, performed best with pyrex glass, and it performed worst with PMMA or acrylic glue materials.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Citation:
Kodzius R, Xiao K, Wu J, Yi X, Gong X, et al. (2012) Inhibitory effect of common microfluidic materials on PCR outcome. Sensors and Actuators B: Chemical 161: 349-358. doi:10.1016/j.snb.2011.10.044.
Publisher:
Elsevier BV
Journal:
Sensors and Actuators B: Chemical
Issue Date:
10-Oct-2013
DOI:
10.1016/j.snb.2011.10.044
Type:
Article
ISSN:
09254005
Sponsors:
Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong Research Grants Council (Grant No. HKUST 603208 and 660207)
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0925400511009361; http://www.sciencedirect.com/science/article/pii/S0925400511009361
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorXiao, Kangen
dc.contributor.authorWu, Jinboen
dc.contributor.authorYi, Xinen
dc.contributor.authorGong, Xiuqingen
dc.contributor.authorFoulds, Ian G.en
dc.contributor.authorWen, Weijiaen
dc.date.accessioned2013-10-10T07:52:48Z-
dc.date.available2013-10-10T07:52:48Z-
dc.date.issued2013-10-10en
dc.identifier.citationKodzius R, Xiao K, Wu J, Yi X, Gong X, et al. (2012) Inhibitory effect of common microfluidic materials on PCR outcome. Sensors and Actuators B: Chemical 161: 349-358. doi:10.1016/j.snb.2011.10.044.en
dc.identifier.issn09254005en
dc.identifier.doi10.1016/j.snb.2011.10.044en
dc.identifier.urihttp://hdl.handle.net/10754/303149en
dc.description.abstractIn this study, we established a simple method for evaluating the PCR compatibility of various common materials employed when fabricating microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most cases, adding bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, although they noticeably interacted with the polymerase. We provide a simple method of performing PCR-compatibility testing of materials using inexpensive instrumentation that is common in molecular biology laboratories. Furthermore, our method is direct, being performed under actual PCR conditions with high temperature. Our results provide an overview of materials that are PCR-friendly for fabricating microfluidic devices. The PCR reaction, without any additives, performed best with pyrex glass, and it performed worst with PMMA or acrylic glue materials.en
dc.description.sponsorshipAward No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong Research Grants Council (Grant No. HKUST 603208 and 660207)en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0925400511009361en
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0925400511009361en
dc.rightsArchived with thanks to Sensors and Actuators B: Chemicalen
dc.subjectBovine serum albumin (BSA)en
dc.subjectMicrofluidicsen
dc.subjectPCR (polymerase chain reaction)en
dc.subjectPCR compatibilityen
dc.subjectPolymeraseen
dc.subjectSurface passivationen
dc.titleInhibitory effect of common microfluidic materials on PCR outcomeen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalSensors and Actuators B: Chemicalen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionThe Hong Kong University of Science and Technology (HKUST)en
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKodzius, Rimantasen
kaust.authorFoulds, Ian G.en
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