Fast detection of genetic information by an optimized PCR in an interchangeable chip.

Handle URI:
http://hdl.handle.net/10754/303136
Title:
Fast detection of genetic information by an optimized PCR in an interchangeable chip.
Authors:
Wu, Jinbo; Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Qin, Jianhua; Wen, Weijia; Xiao, Kang
Abstract:
In this paper, we report the construction of a polymerase chain reaction (PCR) device for fast amplification and detection of DNA. This device consists of an interchangeable PCR chamber, a temperature control component as well as an optical detection system. The DNA amplification happens on an interchangeable chip with the volumes as low as 1.25 μl, while the heating and cooling rate was as fast as 12.7°C/second ensuring that the total time needed of only 25 min to complete the 35 cycle PCR amplification. An optimized PCR with two-temperature approach for denaturing and annealing (Td and Ta) of DNA was also formulated with the PCR chip, with which the amplification of male-specific sex determining region Y (SRY) gene marker by utilizing raw saliva was successfully achieved and the genetic identification was in-situ detected right after PCR by the optical detection system.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Citation:
Wu J, Kodzius R, Xiao K, Qin J, Wen W (2011) Fast detection of genetic information by an optimized PCR in an interchangeable chip. Biomed Microdevices 14: 179-186. doi:10.1007/s10544-011-9595-6.
Publisher:
Springer Nature
Journal:
Biomedical Microdevices
Issue Date:
Feb-2012
DOI:
10.1007/s10544-011-9595-6
PubMed ID:
21976029; 21976029
Type:
Article
ISSN:
1572-8781
Sponsors:
Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong Research Grants Council Grant No. HKUST 603608
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorWu, Jinboen
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorQin, Jianhuaen
dc.contributor.authorWen, Weijiaen
dc.contributor.authorXiao, Kangen
dc.date.accessioned2013-10-10T08:57:40Z-
dc.date.available2013-10-10T08:57:40Z-
dc.date.issued2012-02en
dc.identifier.citationWu J, Kodzius R, Xiao K, Qin J, Wen W (2011) Fast detection of genetic information by an optimized PCR in an interchangeable chip. Biomed Microdevices 14: 179-186. doi:10.1007/s10544-011-9595-6.en
dc.identifier.issn1572-8781en
dc.identifier.pmid21976029en
dc.identifier.pmid21976029en
dc.identifier.doi10.1007/s10544-011-9595-6en
dc.identifier.urihttp://hdl.handle.net/10754/303136en
dc.description.abstractIn this paper, we report the construction of a polymerase chain reaction (PCR) device for fast amplification and detection of DNA. This device consists of an interchangeable PCR chamber, a temperature control component as well as an optical detection system. The DNA amplification happens on an interchangeable chip with the volumes as low as 1.25 μl, while the heating and cooling rate was as fast as 12.7°C/second ensuring that the total time needed of only 25 min to complete the 35 cycle PCR amplification. An optimized PCR with two-temperature approach for denaturing and annealing (Td and Ta) of DNA was also formulated with the PCR chip, with which the amplification of male-specific sex determining region Y (SRY) gene marker by utilizing raw saliva was successfully achieved and the genetic identification was in-situ detected right after PCR by the optical detection system.en
dc.description.sponsorshipAward No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong Research Grants Council Grant No. HKUST 603608en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsArchived with thanks to Biomedical microdevicesen
dc.subjectPoint-of-Care Testingen
dc.subjectMolecular diagnosticsen
dc.subjectPolymerase chain reaction (PCR)en
dc.subjectMicrofluidic Chipen
dc.subjectGenetic identificationen
dc.subject.meshDNAen
dc.subject.meshHumansen
dc.subject.meshMicrofluidic Analytical Techniquesen
dc.subject.meshPoint-of-Care Systemsen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshSalivaen
dc.subject.meshSex-Determining Region Y Proteinen
dc.subject.meshDNAen
dc.subject.meshHumansen
dc.subject.meshMicrofluidic Analytical Techniquesen
dc.subject.meshPoint-of-Care Systemsen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshSalivaen
dc.subject.meshSex-Determining Region Y Proteinen
dc.titleFast detection of genetic information by an optimized PCR in an interchangeable chip.en
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalBiomedical Microdevicesen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionNano Science and Nano Technology program and Physics Department, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.en
dc.contributor.institutionKAUST-HKUST Micro/Nanofluidic Joint Laboratory, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKodzius, Rimantasen

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