The MicroRNA Repertoire of Symbiodinium, the Dinoflagellate Symbiont of Reef-Building Corals

Handle URI:
http://hdl.handle.net/10754/295746
Title:
The MicroRNA Repertoire of Symbiodinium, the Dinoflagellate Symbiont of Reef-Building Corals
Authors:
Baumgarten, Sebastian ( 0000-0003-2646-7699 )
Abstract:
Animal and plant genomes produce numerous small RNAs (smRNAs) that regulate gene expression post-transcriptionally affecting metabolism, development, and epigenetic inheritance. In order to characterize the repertoire of endogenous microRNAs and potential gene targets, we conducted smRNA and mRNA expression profiling over nine experimental treatments of cultures from the dinoflagellate Symbiodinium sp. A1, a photosynthetic symbiont of scleractinian corals. We identified a total of 75 novel smRNAs in Symbiodinum sp. A1 that share stringent key features with functional microRNAs from other model organisms. A subset of 38 smRNAs was predicted independently over all nine treatments and their putative gene targets were identified. We found 3,187 animal-like target sites in the 3’UTRs of 12,858 mRNAs and 53 plantlike target sites in 51,917 genes. Furthermore, we identified the core RNAi protein machinery in Symbiodinium. Integration of smRNA and mRNA expression profiling identified a variety of processes that could be under microRNA control, e.g. regulation of translation, DNA modification, and chromatin silencing. Given that Symbiodinium seems to have a paucity of transcription factors and differentially expressed genes, identification and characterization of its smRNA repertoire establishes the possibility of a range of gene regulatory mechanisms in dinoflagellates acting post-transcriptionally.
Advisors:
Voolstra, Christian R. ( 0000-0003-4555-3795 )
Committee Member:
Aranda, Manuel ( 0000-0001-6673-016X ) ; Pain, Arnab ( 0000-0002-1755-2819 )
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Program:
Marine Science
Issue Date:
Jul-2013
Type:
Thesis
Appears in Collections:
Marine Science Program; Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorVoolstra, Christian R.en
dc.contributor.authorBaumgarten, Sebastianen
dc.date.accessioned2013-07-11T05:25:17Zen
dc.date.available2013-07-11T05:25:17Zen
dc.date.issued2013-07en
dc.identifier.urihttp://hdl.handle.net/10754/295746en
dc.description.abstractAnimal and plant genomes produce numerous small RNAs (smRNAs) that regulate gene expression post-transcriptionally affecting metabolism, development, and epigenetic inheritance. In order to characterize the repertoire of endogenous microRNAs and potential gene targets, we conducted smRNA and mRNA expression profiling over nine experimental treatments of cultures from the dinoflagellate Symbiodinium sp. A1, a photosynthetic symbiont of scleractinian corals. We identified a total of 75 novel smRNAs in Symbiodinum sp. A1 that share stringent key features with functional microRNAs from other model organisms. A subset of 38 smRNAs was predicted independently over all nine treatments and their putative gene targets were identified. We found 3,187 animal-like target sites in the 3’UTRs of 12,858 mRNAs and 53 plantlike target sites in 51,917 genes. Furthermore, we identified the core RNAi protein machinery in Symbiodinium. Integration of smRNA and mRNA expression profiling identified a variety of processes that could be under microRNA control, e.g. regulation of translation, DNA modification, and chromatin silencing. Given that Symbiodinium seems to have a paucity of transcription factors and differentially expressed genes, identification and characterization of its smRNA repertoire establishes the possibility of a range of gene regulatory mechanisms in dinoflagellates acting post-transcriptionally.en
dc.language.isoenen
dc.subjectSymbiodiniumen
dc.subjectmicroRNAen
dc.subjectsmall interfering RNAen
dc.subjectscleractinian coralen
dc.subjectdinoflagellateen
dc.subjectsymbionten
dc.titleThe MicroRNA Repertoire of Symbiodinium, the Dinoflagellate Symbiont of Reef-Building Coralsen
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberAranda, Manuelen
dc.contributor.committeememberPain, Arnaben
thesis.degree.disciplineMarine Scienceen
thesis.degree.nameMaster of Scienceen
dc.person.id121376en
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