Studying Different Clinical Syndromes Of Paediatric Severe Malaria Using Plasma Proteomics

Handle URI:
http://hdl.handle.net/10754/248732
Title:
Studying Different Clinical Syndromes Of Paediatric Severe Malaria Using Plasma Proteomics
Authors:
Ramaprasad, Abhinay ( 0000-0001-9372-5526 )
Abstract:
Background- Severe Plasmodium falciparum malaria remains one of the major causes of childhood morbidity and mortality in Africa. Severe malaria manifests itself as three main clinical syndromes-impaired consciousness (cerebral malaria), respiratory distress and severe malarial anaemia. Cerebral malaria and respiratory distress are major contributors to malaria mortality but their pathophysiology remains unclear. Motivation/Objectives- Most children with severe malaria die within the first 24 hours of admission to a hospital because of their pathophysiological conditions. Thus, along with anti-malarial drugs, various adjuvant therapies such as fluid bolus (for hypovolaemia) and anticonvulsants (for seizures) are given to alleviate the sick child’s condition. But these therapies can sometimes have adverse effects. Hence, a clear understanding of severe malaria pathophysiology is essential for making an informed decision regarding adjuvant therapies. Methodology- We used mass spectrometry-based shotgun proteomics to study plasma samples from Gambian children with severe malaria. We compared the proteomic profiles of different severe malaria syndromes and generated hypotheses regarding the underlying disease mechanisms. Results/Conclusions- The main challenges of studying the severe malaria syndromes using proteomics were the high complexity and variability among the samples. We hypothesized that hepatic injury and nitric oxide play roles in the pathophysiology of cerebral malaria and respiratory distress.
Advisors:
Pain, Arnab ( 0000-0002-1755-2819 )
Committee Member:
Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Casals-Pascual, Climent; Ravasi, Timothy ( 0000-0002-9950-465X )
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Program:
Bioscience
Issue Date:
Aug-2012
Type:
Thesis
Appears in Collections:
Bioscience Program; Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorPain, Arnaben
dc.contributor.authorRamaprasad, Abhinayen
dc.date.accessioned2012-10-14T09:07:37Zen
dc.date.available2012-10-14T09:07:37Zen
dc.date.issued2012-08en
dc.identifier.urihttp://hdl.handle.net/10754/248732en
dc.description.abstractBackground- Severe Plasmodium falciparum malaria remains one of the major causes of childhood morbidity and mortality in Africa. Severe malaria manifests itself as three main clinical syndromes-impaired consciousness (cerebral malaria), respiratory distress and severe malarial anaemia. Cerebral malaria and respiratory distress are major contributors to malaria mortality but their pathophysiology remains unclear. Motivation/Objectives- Most children with severe malaria die within the first 24 hours of admission to a hospital because of their pathophysiological conditions. Thus, along with anti-malarial drugs, various adjuvant therapies such as fluid bolus (for hypovolaemia) and anticonvulsants (for seizures) are given to alleviate the sick child’s condition. But these therapies can sometimes have adverse effects. Hence, a clear understanding of severe malaria pathophysiology is essential for making an informed decision regarding adjuvant therapies. Methodology- We used mass spectrometry-based shotgun proteomics to study plasma samples from Gambian children with severe malaria. We compared the proteomic profiles of different severe malaria syndromes and generated hypotheses regarding the underlying disease mechanisms. Results/Conclusions- The main challenges of studying the severe malaria syndromes using proteomics were the high complexity and variability among the samples. We hypothesized that hepatic injury and nitric oxide play roles in the pathophysiology of cerebral malaria and respiratory distress.en
dc.language.isoenen
dc.subjectMalariaen
dc.subjectPlasma proteomicsen
dc.subjectpediatric malariaen
dc.subjectplasmodium falciparumen
dc.subjectmass spectrometryen
dc.titleStudying Different Clinical Syndromes Of Paediatric Severe Malaria Using Plasma Proteomicsen
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberBajic, Vladimir B.en
dc.contributor.committeememberCasals-Pascual, Climenten
dc.contributor.committeememberRavasi, Timothyen
thesis.degree.disciplineBioscienceen
thesis.degree.nameMaster of Scienceen
dc.person.id113319en
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