Isolation and Purification of Planctomycetes associated with Harbor and Lagoon Seagrasses of the Red Sea

Handle URI:
http://hdl.handle.net/10754/237292
Title:
Isolation and Purification of Planctomycetes associated with Harbor and Lagoon Seagrasses of the Red Sea
Authors:
Bream, Holly
Abstract:
Planctomycetes are members of a unique superphylum along with Verrucomicrobia and Chlamydia, situated in the domain Bacteria. They have distinct structural and morphological features, and discoveries made through phylogenetic analysis indicate their important role in nutrient cycling. Their known relationships with marine photosynthetic organisms led to the formation of this study's hypothesis, namely, that Planctomycetes can be isolated from the biofilm of seagrass species of the Red Sea using cultivation techniques adapted for these organisms. Preparation of solid and liquid media using M13 with both agar and gellan, and 2216 Difco Marine Broth full-­ strength, 1/10-­strength, and with antibiotics, resulted in the successful isolation of Planctomycetes as confirmed by morphological examination and transmission electron microscopy. The work performed in this study provides a solid foundation for further studies to elucidate the metabolic pathways and ecological significance of Planctomycetes.
Advisors:
Bajic, Vladimir B. ( 0000-0001-5435-4750 )
Committee Member:
Lafi, Feras; Saikaly, Pascal E.; Stingl, Ulrich ( 0000-0002-0684-2597 )
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Program:
Bioscience
Issue Date:
Jul-2012
Type:
Thesis
Appears in Collections:
Bioscience Program; Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorBajic, Vladimir B.en
dc.contributor.authorBream, Hollyen
dc.date.accessioned2012-08-05T08:25:44Z-
dc.date.available2012-08-05T08:25:44Z-
dc.date.issued2012-07en
dc.identifier.urihttp://hdl.handle.net/10754/237292en
dc.description.abstractPlanctomycetes are members of a unique superphylum along with Verrucomicrobia and Chlamydia, situated in the domain Bacteria. They have distinct structural and morphological features, and discoveries made through phylogenetic analysis indicate their important role in nutrient cycling. Their known relationships with marine photosynthetic organisms led to the formation of this study's hypothesis, namely, that Planctomycetes can be isolated from the biofilm of seagrass species of the Red Sea using cultivation techniques adapted for these organisms. Preparation of solid and liquid media using M13 with both agar and gellan, and 2216 Difco Marine Broth full-­ strength, 1/10-­strength, and with antibiotics, resulted in the successful isolation of Planctomycetes as confirmed by morphological examination and transmission electron microscopy. The work performed in this study provides a solid foundation for further studies to elucidate the metabolic pathways and ecological significance of Planctomycetes.en
dc.language.isoenen
dc.subjectPlanctomycetesen
dc.subjectCultivationen
dc.subjectPurificationen
dc.subjectSeagrassen
dc.titleIsolation and Purification of Planctomycetes associated with Harbor and Lagoon Seagrasses of the Red Seaen
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberLafi, Ferasen
dc.contributor.committeememberSaikaly, Pascal E.en
dc.contributor.committeememberStingl, Ulrichen
thesis.degree.disciplineBioscienceen
thesis.degree.nameMaster of Scienceen
dc.person.id113250en
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