Diversity of Picoeukaryotes at an Oligotrophic Site off the Northern Red Sea Coast

Handle URI:
http://hdl.handle.net/10754/224071
Title:
Diversity of Picoeukaryotes at an Oligotrophic Site off the Northern Red Sea Coast
Authors:
Espinosa, Francisco Jose Acosta
Abstract:
Picoeukaryotes are protist 3 µm belonging to a wide diversity of taxonomic groups, and they are an important constituent of the ocean microbiota, performing essential ecological roles in marine trophic chains and in nutrient and carbon budgets. Despite this, the true extent of their diversity is currently unknown, and in the last decade molecular surveys have uncovered a substantial number of previously unknown groups from all taxonomic levels. No studies on this group have been done so far on the Red Sea, a unique marine environment characterized by oligotrophic conditions and high irradiance, salinity and water temperature. We sampled the surface waters of a site near the northern Red Sea coast, and analyzed the picoeukaryotic diversity through the construction of PCR clone libraries using the 18S ribosomal gene. The community captured by our library is dominated by three main groups, the alveolates (32%), chlorophytes (32%) and Stramenopiles (20.55%). Members of Radiolaria, Cercozoans and Haptophyta were also found, although in low abundances. Photosynthetic organisms are especially diverse and abundant in the sample, with heterotrophic organism mostly composed by the mostly parasitic novel alveolates and bacterivorous stramenopiles. Novel clades were detected among the Novel Alveolates- II and the photosynthetic stramenopiles taxa, which suggests that they may be part of a number of groups unique to the basin and adapted to the high salinity and temperature conditions. This is the first study done on the Red Sea focusing on the diversity of the complete picoeukaryotic fraction, and provides a stepping stone in the characterization of the picoeukaryotic component of the microbial diversity of the basin.
Advisors:
Stingl, Ulrich ( 0000-0002-0684-2597 )
Committee Member:
Ravasi, Timothy ( 0000-0002-9950-465X ) ; Voolstra, Christian R. ( 0000-0003-4555-3795 )
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Program:
Marine Science
Issue Date:
May-2012
Type:
Thesis
Appears in Collections:
Marine Science Program; Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorStingl, Ulrichen
dc.contributor.authorEspinosa, Francisco Jose Acostaen
dc.date.accessioned2012-05-16T05:49:22Z-
dc.date.available2012-05-16T05:49:22Z-
dc.date.issued2012-05en
dc.identifier.urihttp://hdl.handle.net/10754/224071en
dc.description.abstractPicoeukaryotes are protist 3 µm belonging to a wide diversity of taxonomic groups, and they are an important constituent of the ocean microbiota, performing essential ecological roles in marine trophic chains and in nutrient and carbon budgets. Despite this, the true extent of their diversity is currently unknown, and in the last decade molecular surveys have uncovered a substantial number of previously unknown groups from all taxonomic levels. No studies on this group have been done so far on the Red Sea, a unique marine environment characterized by oligotrophic conditions and high irradiance, salinity and water temperature. We sampled the surface waters of a site near the northern Red Sea coast, and analyzed the picoeukaryotic diversity through the construction of PCR clone libraries using the 18S ribosomal gene. The community captured by our library is dominated by three main groups, the alveolates (32%), chlorophytes (32%) and Stramenopiles (20.55%). Members of Radiolaria, Cercozoans and Haptophyta were also found, although in low abundances. Photosynthetic organisms are especially diverse and abundant in the sample, with heterotrophic organism mostly composed by the mostly parasitic novel alveolates and bacterivorous stramenopiles. Novel clades were detected among the Novel Alveolates- II and the photosynthetic stramenopiles taxa, which suggests that they may be part of a number of groups unique to the basin and adapted to the high salinity and temperature conditions. This is the first study done on the Red Sea focusing on the diversity of the complete picoeukaryotic fraction, and provides a stepping stone in the characterization of the picoeukaryotic component of the microbial diversity of the basin.en
dc.language.isoenen
dc.subjectred seaen
dc.subjectMicrobial Ecologyen
dc.subjectpicoeukaryoticen
dc.subjecteukaryoticen
dc.titleDiversity of Picoeukaryotes at an Oligotrophic Site off the Northern Red Sea Coasten
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberRavasi, Timothyen
dc.contributor.committeememberVoolstra, Christian R.en
thesis.degree.disciplineMarine Scienceen
thesis.degree.nameMaster of Scienceen
dc.person.id113365en
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