Study of the Effect of SRT on Microbial Diversity in Laboratory-scale Sequencing Batch Reactors Using Acclimated and Non-Acclimated Seed

Handle URI:
http://hdl.handle.net/10754/209414
Title:
Study of the Effect of SRT on Microbial Diversity in Laboratory-scale Sequencing Batch Reactors Using Acclimated and Non-Acclimated Seed
Authors:
Tellez, Berenice
Abstract:
Solids Retention Time (SRT) is an important design parameter in activated sludge wastewater treatment systems. In this study, the effect of SRT on the bacterial community structure and diversity was examined in replicate lab-scale activated sludge sequencing batch reactors were operated for a period of 8 weeks and seeded with acclimated or non-acclimated sludge. Four SBRs (acclimated) were set up as duplicates and operated at an SRT of 2 days, and another set of four SBRs (non-acclimated) were operated at an SRT of 10 days. To characterize the microbial community in the SBRs, 16S rRNA gene pyrosequencing was used to measure biodiversity and to assess the reproducibility and stability of the bacterial community structure in replicate reactors. Diversity results showed that SBRs operated at an SRT of 10 days are more diverse than SBRs operated at an SRT of 2 days. This suggests that engineering decision could enhance diversity in activated sludge systems. Cluster analysis based on phylogenetic information revealed that the bacterial community structure was not stable and replicated SBRs evolved differently.
Advisors:
Saikaly, Pascal E.
Committee Member:
Drewes, Jörg E.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Program:
Environmental Science and Engineering
Issue Date:
7-Jul-2011
Type:
Thesis
Appears in Collections:
Environmental Science and Engineering Program; Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorSaikaly, Pascal E.en
dc.contributor.authorTellez, Bereniceen
dc.date.accessioned2012-02-04T08:42:35Z-
dc.date.available2012-02-04T08:42:35Z-
dc.date.issued2011-07-07en
dc.identifier.urihttp://hdl.handle.net/10754/209414en
dc.description.abstractSolids Retention Time (SRT) is an important design parameter in activated sludge wastewater treatment systems. In this study, the effect of SRT on the bacterial community structure and diversity was examined in replicate lab-scale activated sludge sequencing batch reactors were operated for a period of 8 weeks and seeded with acclimated or non-acclimated sludge. Four SBRs (acclimated) were set up as duplicates and operated at an SRT of 2 days, and another set of four SBRs (non-acclimated) were operated at an SRT of 10 days. To characterize the microbial community in the SBRs, 16S rRNA gene pyrosequencing was used to measure biodiversity and to assess the reproducibility and stability of the bacterial community structure in replicate reactors. Diversity results showed that SBRs operated at an SRT of 10 days are more diverse than SBRs operated at an SRT of 2 days. This suggests that engineering decision could enhance diversity in activated sludge systems. Cluster analysis based on phylogenetic information revealed that the bacterial community structure was not stable and replicated SBRs evolved differently.en
dc.language.isoenen
dc.titleStudy of the Effect of SRT on Microbial Diversity in Laboratory-scale Sequencing Batch Reactors Using Acclimated and Non-Acclimated Seeden
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberDrewes, Jörg E.en
thesis.degree.disciplineEnvironmental Science and Engineeringen
thesis.degree.nameMaster of Scienceen
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